BMRB Entry 30087

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PDB ID: 5jwj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30087
MolProbity Validation Chart

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Title:
NMR solution structure of a thermophilic lysine methyl transferase from Sulfolobus islandicus
Deposition date:
2016-05-12
Original release date:
2017-05-19
Authors:
de Lichtenberg, C.; Stiefler-Jensen, D.; Schwarz-Linnet, T.; She, Q.; Teilum, K.
Citation:

Citation: de Lichtenberg, C.; Stiefler-Jensen, D.; Schwarz-Linnet, T.; She, Q.; Teilum, K.. "NMR solution structure of a thermophilic lysine methyl transferase from Sulfolobus islandicus"  .

Assembly members:

Assembly members:
Protein-lysine N-methyltransferase, polymer, 172 residues, 19420.371 Da.

Natural source:

Natural source:   Common Name: crenarchaeotes   Taxonomy ID: 930945   Superkingdom: Archaea   Kingdom: not available   Genus/species: sulfolobus sulfolobus islandicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein-lysine N-methyltransferase: MSYVPHVPYVPTPEKVVRRM LEIAKVSQDDIVYALGCGDG RIIITAAKDFNVKKAVGVEI NDERIREALANIEKNGVTGR ASIVKGNFFEVDISEATVVT MFLLTNVNEMLKPKLEKELK PGTRVVSHEFEIRGWNPKEV IKVEDGNMNHTVYLYVIGEH KAAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts631
15N chemical shifts154
1H chemical shifts1001

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 172 residues - 19420.371 Da.

1   METSERTYRVALPROHISVALPROTYRVAL
2   PROTHRPROGLULYSVALVALARGARGMET
3   LEUGLUILEALALYSVALSERGLNASPASP
4   ILEVALTYRALALEUGLYCYSGLYASPGLY
5   ARGILEILEILETHRALAALALYSASPPHE
6   ASNVALLYSLYSALAVALGLYVALGLUILE
7   ASNASPGLUARGILEARGGLUALALEUALA
8   ASNILEGLULYSASNGLYVALTHRGLYARG
9   ALASERILEVALLYSGLYASNPHEPHEGLU
10   VALASPILESERGLUALATHRVALVALTHR
11   METPHELEULEUTHRASNVALASNGLUMET
12   LEULYSPROLYSLEUGLULYSGLULEULYS
13   PROGLYTHRARGVALVALSERHISGLUPHE
14   GLUILEARGGLYTRPASNPROLYSGLUVAL
15   ILELYSVALGLUASPGLYASNMETASNHIS
16   THRVALTYRLEUTYRVALILEGLYGLUHIS
17   LYSALAALAALALEUGLUHISHISHISHIS
18   HISHIS

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