BMRB Entry 30091

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PDB ID: 5jyt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30091
MolProbity Validation Chart

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Title:
NMR structure of foldswitch-stablized KaiB from Thermosynechococcus elongatus
Deposition date:
2016-05-15
Original release date:
2017-03-23
Authors:
Tseng, Roger; Wang, Andy
Citation:

Citation: Tseng, Roger; Goularte, Nicolette; Chavan, Archana; Luu, Jansen; Cohen, Susan; Chang, Yong-Gang; Heisler, Joel; Li, Sheng; Michael, Alicia; Tripathi, Sarvind; Golden, Susan; LiWang, Andy; Partch, Carrie. "Structural basis of the day-night transition in a bacterial circadian clock"  Science 355, 1174-1180 (2017).
PubMed: 28302851

Assembly members:

Assembly members:
Circadian clock protein KaiB, polymer, 106 residues, 11779.870 Da.

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 197221   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Circadian clock protein KaiB: MAPLRKTAVLKLYVAGNTPN SVRALKTLANILEKEFKGVY ALKVIDVLKNPQLAEEDKIL ATPTLAKVLPPPVRRIIGDL SNREKVLIALRLLAEEIGDY KDDDDK

Data sets:
Data typeCount
13C chemical shifts466
15N chemical shifts97
1H chemical shifts761

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 106 residues - 11779.870 Da.

1   METALAPROLEUARGLYSTHRALAVALLEU
2   LYSLEUTYRVALALAGLYASNTHRPROASN
3   SERVALARGALALEULYSTHRLEUALAASN
4   ILELEUGLULYSGLUPHELYSGLYVALTYR
5   ALALEULYSVALILEASPVALLEULYSASN
6   PROGLNLEUALAGLUGLUASPLYSILELEU
7   ALATHRPROTHRLEUALALYSVALLEUPRO
8   PROPROVALARGARGILEILEGLYASPLEU
9   SERASNARGGLULYSVALLEUILEALALEU
10   ARGLEULEUALAGLUGLUILEGLYASPTYR
11   LYSASPASPASPASPLYS

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