BMRB Entry 30099

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PDB ID: 5k5g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30099
MolProbity Validation Chart

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Title:
Structure of human islet amyloid polypeptide in complex with an engineered binding protein
Deposition date:
2016-05-23
Original release date:
2016-09-22
Authors:
Mirecka, E.; Feuerstein, S.; Gremer, L.; Schroeder, G.; Stoldt, M.; Willbold, D.; Hoyer, W.
Citation:

Citation: Mirecka, E.; Feuerstein, S.; Gremer, L.; Schroeder, G.; Stoldt, M.; Willbold, D.; Hoyer, W.. "beta-Hairpin of Islet Amyloid Polypeptide Bound to an Aggregation Inhibitor"  Sci. Rep. 6, 33474-33474 (2016).
PubMed: 27641459

Assembly members:

Assembly members:
Islet amyloid polypeptide, polymer, 37 residues, 3909.304 Da.
HI18, polymer, 69 residues, 7804.660 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: PET302

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Islet amyloid polypeptide: KCNTATCATQRLANFLVHSS NNFGAILSSTNVGSNTY
HI18: MHHHHHHVNSVDNKFNKEME SAGGEIVYLPNLNPDQLCAF IHSIHDDPSQSANLLAEAKK LNDAQAPKW

Data sets:
Data typeCount
13C chemical shifts320
15N chemical shifts98
1H chemical shifts656

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_2, 12
3entity_2, 22

Entities:

Entity 1, entity_1 37 residues - 3909.304 Da.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSERSER
3   ASNASNPHEGLYALAILELEUSERSERTHR
4   ASNVALGLYSERASNTHRTYR

Entity 2, entity_2, 1 69 residues - 7804.660 Da.

1   METHISHISHISHISHISHISVALASNSER
2   VALASPASNLYSPHEASNLYSGLUMETGLU
3   SERALAGLYGLYGLUILEVALTYRLEUPRO
4   ASNLEUASNPROASPGLNLEUCYSALAPHE
5   ILEHISSERILEHISASPASPPROSERGLN
6   SERALAASNLEULEUALAGLUALALYSLYS
7   LEUASNASPALAGLNALAPROLYSTRP

Download HSQC peak lists in one of the following formats:
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