BMRB Entry 30133

Click here to enlarge.

PDB ID: 5kqj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR30133
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution Structure of Antibiotic-Resistance Factor ANT(2'')-Ia Reveals Substrate-Regulated Conformation Dynamics
Deposition date:
2016-07-06
Original release date:
2017-07-07
Authors:
Bacot-Davis, V.; Berghuis, A.
Citation:

Citation: Bacot-Davis, Valjean; Bassenden, Angelia; Sprules, Tara; Berghuis, Albert. "Effect of solvent and protein dynamics in ligand recognition and inhibition of aminoglycoside adenyltransferase 2"-Ia"  Protein Sci. 26, 1852-1863 (2017).
PubMed: 28734024

Assembly members:

Assembly members:
2''-aminoglycoside nucleotidyltransferase, polymer, 185 residues, 20964.516 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 573   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Klebsiella pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
2''-aminoglycoside nucleotidyltransferase: MDTTQVTLIHKILAAADERN LPLWIGGGWAIDARLGRVTR KHDDIDLTFPGERRGELEAI VEMLGGRVMEELDYGFLAEI GDELLDCEPAWWADEAYEIA EAPQGSCPEAAEGVIAGRPV RCNSWEAIIWDYFYYADEVP PVDWPTKHIESYRLACTSLG AEKVEVLRAAFRSRYAALEH HHHHH

Data sets:
Data typeCount
13C chemical shifts778
15N chemical shifts167
1H chemical shifts1069

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 185 residues - 20964.516 Da.

1   METASPTHRTHRGLNVALTHRLEUILEHIS
2   LYSILELEUALAALAALAASPGLUARGASN
3   LEUPROLEUTRPILEGLYGLYGLYTRPALA
4   ILEASPALAARGLEUGLYARGVALTHRARG
5   LYSHISASPASPILEASPLEUTHRPHEPRO
6   GLYGLUARGARGGLYGLULEUGLUALAILE
7   VALGLUMETLEUGLYGLYARGVALMETGLU
8   GLULEUASPTYRGLYPHELEUALAGLUILE
9   GLYASPGLULEULEUASPCYSGLUPROALA
10   TRPTRPALAASPGLUALATYRGLUILEALA
11   GLUALAPROGLNGLYSERCYSPROGLUALA
12   ALAGLUGLYVALILEALAGLYARGPROVAL
13   ARGCYSASNSERTRPGLUALAILEILETRP
14   ASPTYRPHETYRTYRALAASPGLUVALPRO
15   PROVALASPTRPPROTHRLYSHISILEGLU
16   SERTYRARGLEUALACYSTHRSERLEUGLY
17   ALAGLULYSVALGLUVALLEUARGALAALA
18   PHEARGSERARGTYRALAALALEUGLUHIS
19   HISHISHISHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks