BMRB Entry 30158

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PDB ID: 5t1n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30158
MolProbity Validation Chart

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Title:
Solution-state NMR structural ensemble of NPr (1-85) refined with RDCs and PCS
Deposition date:
2016-08-19
Original release date:
2016-11-14
Authors:
Strickland, M.; Wang, G.; Peterkofsky, A.; Tjandra, N.
Citation:

Citation: Strickland, Madeleine; Stanley, Ann Marie; Wang, Guangshun; Botos, Istvan; Schwieters, Charles; Buchanan, Susan; Peterkofsky, Alan; Tjandra, Nico. "Structure of the NPr:EINNtr Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems"  Structure 24, 2127-2137 (2016).
PubMed: 27839951

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, 9254.570 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 83334   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTVKQTVEITNKLGMHARPA MKLFELMQGFDAEVLLRNDE GTEAEANSVIALLMLDSAKG RQIEVEATGPQEEEALAAVI ALFNS

Data sets:
Data typeCount
13C chemical shifts152
15N chemical shifts74
1H chemical shifts74

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 85 residues - 9254.570 Da.

1   METTHRVALLYSGLNTHRVALGLUILETHR
2   ASNLYSLEUGLYMETHISALAARGPROALA
3   METLYSLEUPHEGLULEUMETGLNGLYPHE
4   ASPALAGLUVALLEULEUARGASNASPGLU
5   GLYTHRGLUALAGLUALAASNSERVALILE
6   ALALEULEUMETLEUASPSERALALYSGLY
7   ARGGLNILEGLUVALGLUALATHRGLYPRO
8   GLNGLUGLUGLUALALEUALAALAVALILE
9   ALALEUPHEASNSER

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