BMRB Entry 30210

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PDB ID: 5u9b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30210
MolProbity Validation Chart

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Title:
Solution structure of the zinc fingers 1 and 2 of MBNL1 in complex with human cardiac troponin T pre-mRNA
Deposition date:
2016-12-15
Original release date:
2017-07-25
Authors:
Phukan, P.; Park, S.; Martinez-Yamout, M.; Zeeb, M.; Dyson, H.; Wright, P.
Citation:

Citation: Park, Sangho; Phukan, Priti Deka; Zeeb, Markus; Martinez-Yamout, Maria; Dyson, Helen Jane; Wright, Peter. "Structural basis for interaction of the tandem zinc finger domains of human muscleblind with cognate RNA from human cardiac troponin T"  Biochemistry 56, 4154-4168 (2017).
PubMed: 28718627

Assembly members:

Assembly members:
Muscleblind-like protein 1, polymer, 92 residues, 10577.180 Da.
RNA (5'-R(P*GP*UP*CP*UP*CP*GP*CP*UP*UP*UP*UP*CP*CP*CP*C)-3'), polymer, 15 residues, 4618.721 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Muscleblind-like protein 1: MAVSVTPIRDTKWLTLEVCR EFQRGTCSRPDTECKFAHPS KSCQVENGRVIACFDSLKGR CSRENCKYLHPPPHLKTQLE INGRNNLIQQKN
RNA (5'-R(P*GP*UP*CP*UP*CP*GP*CP*UP*UP*UP*UP*CP*CP*CP*C)-3'): GUCUCGCUUUUCCCC

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts85
1H chemical shifts554

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3ZINC ION, 13
4ZINC ION, 23

Entities:

Entity 1, entity_1 92 residues - 10577.180 Da.

1   METALAVALSERVALTHRPROILEARGASP
2   THRLYSTRPLEUTHRLEUGLUVALCYSARG
3   GLUPHEGLNARGGLYTHRCYSSERARGPRO
4   ASPTHRGLUCYSLYSPHEALAHISPROSER
5   LYSSERCYSGLNVALGLUASNGLYARGVAL
6   ILEALACYSPHEASPSERLEULYSGLYARG
7   CYSSERARGGLUASNCYSLYSTYRLEUHIS
8   PROPROPROHISLEULYSTHRGLNLEUGLU
9   ILEASNGLYARGASNASNLEUILEGLNGLN
10   LYSASN

Entity 2, entity_2 15 residues - 4618.721 Da.

1   GUCUCGCUUU
2   UCCCC

Entity 3, ZINC ION, 1 - 65.409 Da.

1   ZNZN

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