BMRB Entry 30305

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PDB ID: 5w4s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30305
MolProbity Validation Chart

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Title:
Solution structure of C2 domain from protein kinase C alpha in ternary complex with calcium and V5-pHM peptide
Deposition date:
2017-06-12
Original release date:
2018-04-20
Authors:
Yang, Y.; Igumenova, T.
Citation:

Citation: Yang, Yuan; Shu, Chang; Li, Pingwei; Igumenova, Tatyana. "Structural basis of protein kinase C alpha regulation by the C-terminal tail"  Biophys. J. 114, 1590-1603 (2018).
PubMed: 29642029

Assembly members:

Assembly members:
Protein kinase C alpha type, polymer, 139 residues, 16240.451 Da.
V5-pHM peptide, polymer, 12 residues, 1298.187 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein kinase C alpha type: HTEKRGRIYLKAEVTDEKLH VTVRDAKNLIPMDPNGLSDP YVKLKLIPDPKNESKQKTKT IRSTLNPQWNESFTFKLKPS DKDRRLSVEIWDWDRTTRND FMGSLSFGVSELMKMPASGW YKLLNQEEGEYYNVPIPEG
V5-pHM peptide: XDQSDFEGFXYX

Data sets:
Data typeCount
1H chemical shifts59

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3entity_3, 13
4entity_3, 23

Entities:

Entity 1, entity_1 139 residues - 16240.451 Da.

1   HISTHRGLULYSARGGLYARGILETYRLEU
2   LYSALAGLUVALTHRASPGLULYSLEUHIS
3   VALTHRVALARGASPALALYSASNLEUILE
4   PROMETASPPROASNGLYLEUSERASPPRO
5   TYRVALLYSLEULYSLEUILEPROASPPRO
6   LYSASNGLUSERLYSGLNLYSTHRLYSTHR
7   ILEARGSERTHRLEUASNPROGLNTRPASN
8   GLUSERPHETHRPHELYSLEULYSPROSER
9   ASPLYSASPARGARGLEUSERVALGLUILE
10   TRPASPTRPASPARGTHRTHRARGASNASP
11   PHEMETGLYSERLEUSERPHEGLYVALSER
12   GLULEUMETLYSMETPROALASERGLYTRP
13   TYRLYSLEULEUASNGLNGLUGLUGLYGLU
14   TYRTYRASNVALPROILEPROGLUGLY

Entity 2, entity_2 12 residues - 1298.187 Da.

1   ACEASPGLNSERASPPHEGLUGLYPHESEP
2   TYRNH2

Entity 3, entity_3, 1 - Ca - 40.078 Da.

1   CA