BMRB Entry 30345

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PDB ID: 6b1g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30345
MolProbity Validation Chart

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Title:
Solution structure of TDP-43 N-terminal domain dimer.
Deposition date:
2017-09-18
Original release date:
2018-02-21
Authors:
Naik, M.; Wang, A.; Conicella, A.; Fawzi, N.
Citation:

Citation: Wang, Ailin; Conicella, Alexander; Schmidt, Hermann Broder; Martin, Erik; Rhoads, Shannon; Reeb, Ashley; Nourse, Amanda; Ramirez Montero, Daniel; Ryan, Veronica; Rohatgi, Rajat; Shewmaker, Frank; Naik, Mandar; Mittag, Tanja; Ayala, Yuna; Fawzi, Nicolas. "A single N-terminal phosphomimic disrupts TDP-43 polymerization, phase separation and RNA splicing"  EMBO J. 37, e97452-e97452 (2018).
PubMed: 29438978

Assembly members:

Assembly members:
entity_1, polymer, 83 residues, 9244.335 Da.
entity_2, polymer, 83 residues, 9196.318 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411/TDP-43 S48E

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMMSEYIRVTEDENDEPIE IPSEDDGTVLLSTVTAQFPG ACGLRYRNPVEQCMRGVRLV EGILHAPDAGWGNLVYVVNY PKD
entity_2: GHMMSERIRVTEDENDEPIE IPSEDDGTVLLSTVTAQFPG ACGLRYRNPVSQCMRGVRLV EGILHAPDAGWGNLVYVVNY PKD

Data sets:
Data typeCount
13C chemical shifts654
15N chemical shifts161
1H chemical shifts1080

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 83 residues - 9244.335 Da.

1   GLYHISMETMETSERGLUTYRILEARGVAL
2   THRGLUASPGLUASNASPGLUPROILEGLU
3   ILEPROSERGLUASPASPGLYTHRVALLEU
4   LEUSERTHRVALTHRALAGLNPHEPROGLY
5   ALACYSGLYLEUARGTYRARGASNPROVAL
6   GLUGLNCYSMETARGGLYVALARGLEUVAL
7   GLUGLYILELEUHISALAPROASPALAGLY
8   TRPGLYASNLEUVALTYRVALVALASNTYR
9   PROLYSASP

Entity 2, entity_2 83 residues - 9196.318 Da.

1   GLYHISMETMETSERGLUARGILEARGVAL
2   THRGLUASPGLUASNASPGLUPROILEGLU
3   ILEPROSERGLUASPASPGLYTHRVALLEU
4   LEUSERTHRVALTHRALAGLNPHEPROGLY
5   ALACYSGLYLEUARGTYRARGASNPROVAL
6   SERGLNCYSMETARGGLYVALARGLEUVAL
7   GLUGLYILELEUHISALAPROASPALAGLY
8   TRPGLYASNLEUVALTYRVALVALASNTYR
9   PROLYSASP

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