BMRB Entry 30680

Title:
Sleeping Beauty transposase PAI subdomain mutant - H19Y
Deposition date:
2019-10-24
Original release date:
2020-10-23
Authors:
Nesmelova, I.; Leighton, G.; Yan, C.; Lustig, J.; Corona, R.; Guo, J.; Ivics, Z.
Citation:

Citation: Nesmelova, I.; Leighton, G.; Yan, C.; Lustig, J.; Corona, R.; Guo, J.; Ivics, Z.. "H19Y mutation in the primary DNA-recognition subdomain of the Sleeping Beauty transposase improves structural stability, transposon DNA-binding and transposition"  .

Assembly members:

Assembly members:
entity_1, polymer, 57 residues, 6389.402 Da.

Natural source:

Natural source:   Common Name: Rainbow trout   Taxonomy ID: 8022   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oncorhynchus mykiss

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts143
15N chemical shifts55
1H chemical shifts360

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 57 residues - 6389.402 Da.

1   ALASERMETGLYLYSSERLYSGLUILESER
2   GLNASPLEUARGLYSARGILEVALASPLEU
3   TYRLYSSERGLYSERSERLEUGLYALAILE
4   SERLYSARGLEUALAVALPROARGSERSER
5   VALGLNTHRILEVALARGLYSTYRLYSHIS
6   HISGLYTHRTHRGLNHISHIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks