BMRB Entry 30832
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30832
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Title: ApoL1 N-terminal domain PubMed: 34316015
Deposition date: 2020-12-23 Original release date: 2021-08-02
Authors: Holliday, M.; Ultsch, M.; Moran, P.; Fairbrother, W.; Kirchhofer, D.
Citation: Ultsch, Mark; Holliday, Michael; Gerhardy, Stefan; Moran, Paul; Scales, Suzie; Gupta, Nidhi; Oltrabella, Francesca; Chiu, Cecilia; Fairbrother, Wayne; Eigenbrot, Charles; Kirchhofer, Daniel. "Structures of the ApoL1 and ApoL2 N-terminal domains reveal a novel structural motif" Commun. Biol. 4, 916-916 (2021).
Assembly members:
entity_1, polymer, 114 residues, 13309.070 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: GSDPESSIFIEDAIKYFKEK
VSTQNLLLLLTDNEAWNGFV
AAAELPRNEADELRKALDNL
ARQMIMKDKNWHDKGQQYRN
WFLKEFPRLKSELEDNIRRL
RALADGVQKVHKGT
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 466 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 726 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 114 residues - 13309.070 Da.
| 1 | GLY | SER | ASP | PRO | GLU | SER | SER | ILE | PHE | ILE | ||||
| 2 | GLU | ASP | ALA | ILE | LYS | TYR | PHE | LYS | GLU | LYS | ||||
| 3 | VAL | SER | THR | GLN | ASN | LEU | LEU | LEU | LEU | LEU | ||||
| 4 | THR | ASP | ASN | GLU | ALA | TRP | ASN | GLY | PHE | VAL | ||||
| 5 | ALA | ALA | ALA | GLU | LEU | PRO | ARG | ASN | GLU | ALA | ||||
| 6 | ASP | GLU | LEU | ARG | LYS | ALA | LEU | ASP | ASN | LEU | ||||
| 7 | ALA | ARG | GLN | MET | ILE | MET | LYS | ASP | LYS | ASN | ||||
| 8 | TRP | HIS | ASP | LYS | GLY | GLN | GLN | TYR | ARG | ASN | ||||
| 9 | TRP | PHE | LEU | LYS | GLU | PHE | PRO | ARG | LEU | LYS | ||||
| 10 | SER | GLU | LEU | GLU | ASP | ASN | ILE | ARG | ARG | LEU | ||||
| 11 | ARG | ALA | LEU | ALA | ASP | GLY | VAL | GLN | LYS | VAL | ||||
| 12 | HIS | LYS | GLY | THR |
Samples:
sample_1: ApoL1 (61-172), [U-99% 13C; U-99% 15N], 0.9 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CC(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C TROSY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
CYANA v3.97, Guntert, Mumenthaler and Wuthrich - structure calculation
CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE 800 MHz
- Bruker AVANCE 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts