BMRB Entry 30950

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PDB ID: 7s5j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30950
MolProbity Validation Chart

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Title:
Solution NMR structure of substrate bound peptidase domain from PCAT1
Deposition date:
2021-09-10
Original release date:
2021-12-31
Authors:
Bhattacharya, S.; Palillo, A.
Citation:

Citation: Bhattacharya, S.; Palillo, A.. "Structural and dynamic studies of the peptidase domain from Clostridium thermocellum PCAT1"  Protein Sci. 31, 498-512 (2022).
PubMed: 34865273

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, 16635.389 Da.
entity_2, polymer, 24 residues, 2672.960 Da.

Natural source:

Natural source:   Common Name: Acetivibrio thermocellus   Taxonomy ID: 203119   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Acetivibrio thermocellus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PMCSG20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNAMLRRLFKKKYVCVRQYD LTDAGAACLSSIAQYYGLKM SLAKIREMTGTDTQGTNAYG LIHAAKQLGFSAKGVKASKE DLLKDFRLPAIANVIVDNRL AHFVVIYSIKNRIITVADPG KGIVRYSMDDFCSIWTGGLV LLEPGEAFQKG
entity_2: LNIGRELTDEELMEMTGGST FSIQ

Data sets:
Data typeCount
13C chemical shifts633
15N chemical shifts154
1H chemical shifts1021

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 151 residues - 16635.389 Da.

1   SERASNALAMETLEUARGARGLEUPHELYS
2   LYSLYSTYRVALCYSVALARGGLNTYRASP
3   LEUTHRASPALAGLYALAALACYSLEUSER
4   SERILEALAGLNTYRTYRGLYLEULYSMET
5   SERLEUALALYSILEARGGLUMETTHRGLY
6   THRASPTHRGLNGLYTHRASNALATYRGLY
7   LEUILEHISALAALALYSGLNLEUGLYPHE
8   SERALALYSGLYVALLYSALASERLYSGLU
9   ASPLEULEULYSASPPHEARGLEUPROALA
10   ILEALAASNVALILEVALASPASNARGLEU
11   ALAHISPHEVALVALILETYRSERILELYS
12   ASNARGILEILETHRVALALAASPPROGLY
13   LYSGLYILEVALARGTYRSERMETASPASP
14   PHECYSSERILETRPTHRGLYGLYLEUVAL
15   LEULEUGLUPROGLYGLUALAPHEGLNLYS
16   GLY

Entity 2, unit_2 24 residues - 2672.960 Da.

1   LEUASNILEGLYARGGLULEUTHRASPGLU
2   GLULEUMETGLUMETTHRGLYGLYSERTHR
3   PHESERILEGLN

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