BMRB Entry 31040
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31040
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Title: Solution structure of the WH domain of MORF
Deposition date: 2022-08-19 Original release date: 2023-05-02
Authors: Zhang, Y.; Kutateladze, T.
Citation: Klein, B.; Zhang, Y.; Kutateladze, T.. "Structure of MORF-WH" .
Assembly members:
entity_1, polymer, 84 residues, 9515.630 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: GNDLRNVDWNKLLRRAIEGL
EEPNGSSLKNIEKYLRSQSD
LTSTTNNPAFQQRLRLGAKR
AVNNGRLLKDGPQYRVNYGS
LDGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 459 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 84 residues - 9515.630 Da.
| 1 | GLY | ASN | ASP | LEU | ARG | ASN | VAL | ASP | TRP | ASN | ||||
| 2 | LYS | LEU | LEU | ARG | ARG | ALA | ILE | GLU | GLY | LEU | ||||
| 3 | GLU | GLU | PRO | ASN | GLY | SER | SER | LEU | LYS | ASN | ||||
| 4 | ILE | GLU | LYS | TYR | LEU | ARG | SER | GLN | SER | ASP | ||||
| 5 | LEU | THR | SER | THR | THR | ASN | ASN | PRO | ALA | PHE | ||||
| 6 | GLN | GLN | ARG | LEU | ARG | LEU | GLY | ALA | LYS | ARG | ||||
| 7 | ALA | VAL | ASN | ASN | GLY | ARG | LEU | LEU | LYS | ASP | ||||
| 8 | GLY | PRO | GLN | TYR | ARG | VAL | ASN | TYR | GLY | SER | ||||
| 9 | LEU | ASP | GLY | LYS |
Samples:
sample_1: MORF-WH, [U-13C; U-15N], 1.33 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation
CcpNmr Analysis, CCPN - chemical shift assignment
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker AVANCE 600 MHz
- Varian INOVA 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts