BMRB Entry 31076
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31076
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Title: Taipan Natriuretic Peptide C -TNPc
Deposition date: 2023-03-30 Original release date: 2023-04-10
Authors: Torres, A.; Alewood, P.
Citation: Vink, Simone; Akondi, Kalyana; Jin, Jean; Poth, Kim; Torres, Allan; Kuchel, Philip; Burke, Sandra; Head, Geoffrey; Alewood, Paul. "Taipan Natriuretic Peptides Are Potent and Selective Agonists for the Natriuretic Peptide Receptor A" Molecules 28, 3063-3063 (2023).
Assembly members:
entity_1, polymer, 39 residues, 4119.686 Da.
Natural source: Common Name: Oxyuranus microlepidotus Taxonomy ID: 111177 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oxyuranus microlepidotus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: SDSKIGNGCFGFPLDRIGSV
SGLGCNRIMQNPPKKFSGE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 193 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 39 residues - 4119.686 Da.
| 1 | SER | ASP | SER | LYS | ILE | GLY | ASN | GLY | CYS | PHE | ||||
| 2 | GLY | PHE | PRO | LEU | ASP | ARG | ILE | GLY | SER | VAL | ||||
| 3 | SER | GLY | LEU | GLY | CYS | ASN | ARG | ILE | MET | GLN | ||||
| 4 | ASN | PRO | PRO | LYS | LYS | PHE | SER | GLY | GLU |
Samples:
sample_1: Taipan Natriuetic Peptide C -TNPc 1 mM
sample_conditions_1: pH: 4.4; pressure: 1 atm; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
DYANA, Guntert, Braun and Wuthrich - structure calculation
XEASY, Bartels et al. - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker DMX 600 MHz