BMRB Entry 34061

Name: Second zinc-binding domain from yeast Pcf11
Published: 2017-07-26

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PDB ID: 5m9z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34061
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Second zinc-binding domain from yeast Pcf11

Deposition date:

2016-11-02

Original release date:

2017-07-26

Authors:

Mackereth, C.

Citation:

Citation: Guegueniat, Julia; Dupin, Adrien; Stojko, Johan; Beaurepaire, Lionel; Cianferani, Sarah; Mackereth, Cameron; Minvielle-Sebastia, Lionel; Fribourg, Sebastien. "Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing." Nucleic Acids Res. 45, 10115-10131 (2017).
PubMed: 28973460

Assembly members:

Assembly members:
Protein PCF11, polymer, 97 residues, 10838.987 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21 Vector: pET-His1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein PCF11: GSVDNTLGSDRSNELEIRGK YVVVPETSQDMAFKCPICKE TVTGVYDEESGEWVWKNTIE VNGKYFHSTCYHETSQNSSK SNSGKVGLDDLKKLVTK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	228
15N chemical shifts	52
1H chemical shifts	352

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION	2

Entities:

Entity 1, entity_1 97 residues - 10838.987 Da.

1

GLY

SER

VAL

ASP

ASN

THR

LEU

GLY

SER

ASP

2

ARG

SER

ASN

GLU

LEU

GLU

ILE

ARG

GLY

LYS

3

TYR

VAL

PRO

GLU

THR

SER

GLN

ASP

4

MET

ALA

PHE

LYS

CYS

PRO

ILE

CYS

LYS

GLU

5

THR

VAL

THR

GLY

VAL

TYR

ASP

GLU

SER

6

GLY

GLU

TRP

VAL

TRP

LYS

ASN

THR

ILE

GLU

7

VAL

ASN

GLY

LYS

TYR

PHE

HIS

SER

THR

CYS

8

TYR

HIS

GLU

THR

SER

GLN

ASN

SER

LYS

9

SER

ASN

SER

GLY

LYS

VAL

GLY

LEU

ASP

10

LEU

LYS

LEU

VAL

THR

LYS

Entity 2, ZINC ION - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCACO	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HNHA	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
3D H(C)CH-TOCSY	sample_4	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC CT	sample_5	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HMBC	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 34061

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: