BMRB Entry 34087
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PDB ID: 5mwq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34087
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Stadnik, D.; Bierczynska-Krzysik, A.; Zielinska, J.; Antosik, J.; Borowicz, P.; Bocian, W.; Sitkowski, J.; Bednarek, E.; Kozerski, L.. "Identification of Lysine Misincorporation at Asparagine Position in Recombinant Insulin Analogs Produced in E. coli" Pharm. Res. 36, 79-79 (2019).
PubMed: 30949841
Assembly members:
Insulin, polymer, 21 residues, 2398.775 Da.
Insulin, polymer, 32 residues, 3720.326 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Insulin: GIVEQCCTSICSLYQLENYC
K
Insulin: FVNQHLCGSHLVEALYLVCG
ERGFFYTPKTKR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 361 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 21 residues - 2398.775 Da.
| 1 | GLY | ILE | VAL | GLU | GLN | CYS | CYS | THR | SER | ILE | ||||
| 2 | CYS | SER | LEU | TYR | GLN | LEU | GLU | ASN | TYR | CYS | ||||
| 3 | LYS |
Entity 2, entity_2 32 residues - 3720.326 Da.
| 1 | PHE | VAL | ASN | GLN | HIS | LEU | CYS | GLY | SER | HIS | ||||
| 2 | LEU | VAL | GLU | ALA | LEU | TYR | LEU | VAL | CYS | GLY | ||||
| 3 | GLU | ARG | GLY | PHE | PHE | TYR | THR | PRO | LYS | THR | ||||
| 4 | LYS | ARG |