BMRB Entry 34114

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PDB ID: 5nda
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR34114
MolProbity Validation Chart

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Title:
NMR Structural Characterisation of Pharmaceutically Relevant Proteins Obtained Through a Novel Recombinant Production: The Case of The Pulmonary Surfactant Polypeptide C Analogue rSP-C33Leu.
Deposition date:
2017-03-08
Original release date:
2017-06-02
Authors:
Venturi, L.; Pioselli, B.; Johansson, J.; Rising, A.; Kronqvist, N.; Nordling, K.
Citation:

Citation: Kronqvist, N.; Sarr, M.; Lindqvist, A.; Nordling, K.; Otikovs, M.; Venturi, L.; Pioselli, B.; Purhonen, P.; Landreh, M.; Biverstal, H.; Toleikis, Z.; Sjoberg, L.; Robinson, C.; Pelizzi, N.; Jornvall, H.; Hebert, H.; Jaudzems, K.; Curstedt, T.; Rising, A.; Johansson, J.. "Efficient protein production inspired by how spiders make silk"  Nat. Commun. 8, 15504-15504 (2017).
PubMed: 28534479

Assembly members:

Assembly members:
entity_1, polymer, 33 residues, 3600.728 Da.

Natural source:

Natural source:   Common Name: Pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IPSSPVHLKRLKLLLLLLLL ILLLILGALLLGL

Data sets:
Data typeCount
13C chemical shifts41
15N chemical shifts31
1H chemical shifts295

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 33 residues - 3600.728 Da.

1   ILEPROSERSERPROVALHISLEULYSARG
2   LEULYSLEULEULEULEULEULEULEULEU
3   ILELEULEULEUILELEUGLYALALEULEU
4   LEUGLYLEU

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