BMRB Entry 34120

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PDB ID: 5nko
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34120
MolProbity Validation Chart

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Title:
Solution structure of the C-terminal domain of S. aureus Hibernating Promoting Factor (CTD-SaHPF)
Deposition date:
2017-03-31
Original release date:
2017-06-30
Authors:
Usachev, K.; Khusainov, I.; Ayupov, R.; Validov, S.; Kieffer, B.; Yusupov, M.
Citation:

Citation: Khusainov, Iskander; Vicens, Quentin; Ayupov, Rustam; Usachev, Konstantin; Myasnikov, Alexander; Simonetti, Angelita; Validov, Shamil; Kieffer, Bruno; Yusupova, Gulnara; Yusupov, Marat; Hashem, Yaser. "Structures and dynamics of hibernating ribosomes from Staphylococcus aureus mediated by intermolecular interactions of HPF"  EMBO J. 36, 2073-2087 (2017).
PubMed: 28645916

Assembly members:

Assembly members:
entity_1, polymer, 61 residues, 7124.070 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 93061   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pGS21A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MIEIIRSKEFSLKPMDSEEA VLQMNLLGHDFFVFTDRETD GTSIVYRRKDGKYGLIQTSE Q

Data sets:
Data typeCount
13C chemical shifts516
15N chemical shifts120
1H chemical shifts760

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21

Entities:

Entity 1, entity_1, 1 61 residues - 7124.070 Da.

1   METILEGLUILEILEARGSERLYSGLUPHE
2   SERLEULYSPROMETASPSERGLUGLUALA
3   VALLEUGLNMETASNLEULEUGLYHISASP
4   PHEPHEVALPHETHRASPARGGLUTHRASP
5   GLYTHRSERILEVALTYRARGARGLYSASP
6   GLYLYSTYRGLYLEUILEGLNTHRSERGLU
7   GLN

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