BMRB Entry 34282

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PDB ID: 6grv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34282
MolProbity Validation Chart

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Title:
Cadmium(II) form of full-length metallothionein from Pseudomonas fluorescens Q2-87 (PflQ2 MT)
Deposition date:
2018-06-12
Original release date:
2018-09-13
Authors:
Habjanic, J.; Zerbe, O.; Freisinger, E.
Citation:

Citation: Habjanic, J.; Zerbe, O.; Freisinger, E.. "A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc."  Metallomics 10, 1415-1429 (2018).
PubMed: 30191219

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 8822.591 Da.
entity_CD, non-polymer, 112.411 Da.

Natural source:

Natural source:   Common Name: Pseudomonas fluorescens Q2-87   Taxonomy ID: 1038922   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas fluorescens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NELRCGCPDCHCKVDPERVF NHDGEAYCSQACAEQHPNGE PCPAPDCHCERSGKVGGRDI TNNQLDEALEETFPASDPIS P

Data typeCount
13C chemical shifts309
15N chemical shifts85
1H chemical shifts489

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_2, 12
3entity_2, 22
4entity_2, 32
5entity_2, 42

Entities:

Entity 1, entity_1 81 residues - 8822.591 Da.

1   ASNGLULEUARGCYSGLYCYSPROASPCYS
2   HISCYSLYSVALASPPROGLUARGVALPHE
3   ASNHISASPGLYGLUALATYRCYSSERGLN
4   ALACYSALAGLUGLNHISPROASNGLYGLU
5   PROCYSPROALAPROASPCYSHISCYSGLU
6   ARGSERGLYLYSVALGLYGLYARGASPILE
7   THRASNASNGLNLEUASPGLUALALEUGLU
8   GLUTHRPHEPROALASERASPPROILESER
9   PRO

Entity 2, entity_2, 1 - Cd - 112.411 Da.

1   CD

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