BMRB Entry 34304

Click here to enlarge.

PDB ID: 6h5h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34304
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
A computationally designed dRP lyase domain reconstructed from two heterologous fragments
Deposition date:
2018-07-24
Original release date:
2018-12-05
Authors:
ElGamacy, M.; Coles, M.; Lupas, A.
Citation:

Citation: ElGamacy, M.; Coles, M.; Lupas, A.. "Asymmetric protein design from conserved supersecondary structures"  J. Struct. Biol. 204, 380-387 (2018).
PubMed: 30558718

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 13443.259 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKHHHHHHPMSDYDIPTTEN LYFQGAMGGQETLNGALVNM LKEEGNKALSVGNIDDALQY YAAAITLDKYPHKIKSGAEA KKLPGVGTKIAEKIDEFLAT GKLRKLEKIRQDDTSSSINF L

Data sets:
Data typeCount
13C chemical shifts257
15N chemical shifts61
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 121 residues - 13443.259 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   SERASPTYRASPILEPROTHRTHRGLUASN
3   LEUTYRPHEGLNGLYALAMETGLYGLYGLN
4   GLUTHRLEUASNGLYALALEUVALASNMET
5   LEULYSGLUGLUGLYASNLYSALALEUSER
6   VALGLYASNILEASPASPALALEUGLNTYR
7   TYRALAALAALAILETHRLEUASPLYSTYR
8   PROHISLYSILELYSSERGLYALAGLUALA
9   LYSLYSLEUPROGLYVALGLYTHRLYSILE
10   ALAGLULYSILEASPGLUPHELEUALATHR
11   GLYLYSLEUARGLYSLEUGLULYSILEARG
12   GLNASPASPTHRSERSERSERILEASNPHE
13   LEU

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks