BMRB Entry 34428

Name: Mouse RBM20 RRM domain in complex with AUCUUA RNA
Published: 2019-11-01

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PDB ID: 6so9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34428
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Mouse RBM20 RRM domain in complex with AUCUUA RNA

Deposition date:

2019-08-29

Original release date:

2019-11-01

Authors:

Mackereth, C.; Upadhyay, S.

Citation:

Citation: Upadhyay, Santosh Kumar; Mackereth, Cameron. "Structural basis of UCUU RNA motif recognition by splicing factor RBM20" Nucleic Acids Res. 48, 4538-4550 (2020).
PubMed: 32187365

Assembly members:

Assembly members:
entity_1, polymer, 6 residues, 1757.153 Da.
entity_2, polymer, 112 residues, 12571.588 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XUCUUA
entity_2: GAMAQRKGAGRVVHICNLPE GSCTENDVINLGLPFGKVTN YILMKSTNQAFLEMAYTEAA QAMVQYYQEKPAIINGEKLL IRMSTRYKELQLKKPGKNVA AIIQDIHSQRER

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	501
15N chemical shifts	123
1H chemical shifts	854

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 6 residues - 1757.153 Da.

1

ADN

U

C

U

A

Entity 2, entity_2 112 residues - 12571.588 Da.

1

GLY

ALA

MET

ALA

GLN

ARG

LYS

GLY

ALA

GLY

2

ARG

VAL

HIS

ILE

CYS

ASN

LEU

PRO

GLU

3

GLY

SER

CYS

THR

GLU

ASN

ASP

VAL

ILE

ASN

4

LEU

GLY

LEU

PRO

PHE

GLY

LYS

VAL

THR

ASN

5

TYR

ILE

LEU

MET

LYS

SER

THR

ASN

GLN

ALA

6

PHE

LEU

GLU

MET

ALA

TYR

THR

GLU

ALA

7

GLN

ALA

MET

VAL

GLN

TYR

GLN

GLU

LYS

8

PRO

ALA

ILE

ASN

GLY

GLU

LYS

LEU

9

ILE

ARG

MET

SER

THR

ARG

TYR

LYS

GLU

LEU

10

GLN

LEU

LYS

PRO

GLY

LYS

ASN

VAL

ALA

11

ALA

ILE

GLN

ASP

ILE

HIS

SER

GLN

ARG

12

GLU

ARG

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_5	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC CT	sample_6	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_5	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_5	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_4	isotropic	sample_conditions_1
2D x2-filtered 1H-1H NOESY	sample_7	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_4	isotropic	sample_conditions_1
2D x2-filtered 1H-1H NOESY	sample_5	isotropic	sample_conditions_1
3D (H)C(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_6	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 34428

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: