BMRB Entry 34429

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PDB ID: 6soe
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34429
MolProbity Validation Chart

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Title:
Mouse RBM20 RRM domain
Deposition date:
2019-08-29
Original release date:
2019-11-01
Authors:
Mackereth, C.; Upadhyay, S.
Citation:

Citation: Upadhyay, Santosh Kumar; Mackereth, Cameron. "Structural basis of UCUU RNA motif recognition by splicing factor RBM20"  Nucleic Acids Res. 48, 4538-4550 (2020).
PubMed: 32187365

Assembly members:

Assembly members:
entity_1, polymer, 112 residues, 12571.588 Da.

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET-His1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMAQRKGAGRVVHICNLPE GSCTENDVINLGLPFGKVTN YILMKSTNQAFLEMAYTEAA QAMVQYYQEKPAIINGEKLL IRMSTRYKELQLKKPGKNVA AIIQDIHSQRER

Data sets:
Data typeCount
13C chemical shifts518
15N chemical shifts119
1H chemical shifts821

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 112 residues - 12571.588 Da.

1   GLYALAMETALAGLNARGLYSGLYALAGLY
2   ARGVALVALHISILECYSASNLEUPROGLU
3   GLYSERCYSTHRGLUASNASPVALILEASN
4   LEUGLYLEUPROPHEGLYLYSVALTHRASN
5   TYRILELEUMETLYSSERTHRASNGLNALA
6   PHELEUGLUMETALATYRTHRGLUALAALA
7   GLNALAMETVALGLNTYRTYRGLNGLULYS
8   PROALAILEILEASNGLYGLULYSLEULEU
9   ILEARGMETSERTHRARGTYRLYSGLULEU
10   GLNLEULYSLYSPROGLYLYSASNVALALA
11   ALAILEILEGLNASPILEHISSERGLNARG
12   GLUARG

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