BMRB Entry 34439

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PDB ID: 6szc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34439
MolProbity Validation Chart

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Title:
NMR structure of repeat domain 13 of the fibrillar adhesin CshA from Streptococcus gordonii.
Deposition date:
2019-10-02
Original release date:
2020-04-03
Authors:
Higman, V.; Back, C.; Crump, M.; Race, P.
Citation:

Citation: Back, C.; Higman, V.; LeVay, K.; Patel, V.; Parnell, A.; Frankel, D.; Jenkinson, H.; Burston, S.; Crump, M.; Nobbs, A.; Race, P.. "The streptococcal multidomain fibrillar adhesin CshA has an elongated polymeric architecture"  J. Biol. Chem. 295, 6689-6699 (2020).
PubMed: 32229583

Assembly members:

Assembly members:
Surface-associated protein CshA, polymer, 118 residues, 12480.738 Da.

Natural source:

Natural source:   Common Name: Streptococcus gordonii   Taxonomy ID: 467705   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus gordonii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pOPINF

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Surface-associated protein CshA: MAHHHHHHSSGLEVLFQGPT GTGATSTGPQGLPQTGTPTF QGGDPLVPIDETVEPTFEDG SKEKTIPGQGTYTIVPDGTV TFTPDKQFVGKPDPVTVKRV DKNGTPVTATYSPEFTKV

Data sets:
Data typeCount
13C chemical shifts309
15N chemical shifts61
1H chemical shifts483

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 118 residues - 12480.738 Da.

1   METALAHISHISHISHISHISHISSERSER
2   GLYLEUGLUVALLEUPHEGLNGLYPROTHR
3   GLYTHRGLYALATHRSERTHRGLYPROGLN
4   GLYLEUPROGLNTHRGLYTHRPROTHRPHE
5   GLNGLYGLYASPPROLEUVALPROILEASP
6   GLUTHRVALGLUPROTHRPHEGLUASPGLY
7   SERLYSGLULYSTHRILEPROGLYGLNGLY
8   THRTYRTHRILEVALPROASPGLYTHRVAL
9   THRPHETHRPROASPLYSGLNPHEVALGLY
10   LYSPROASPPROVALTHRVALLYSARGVAL
11   ASPLYSASNGLYTHRPROVALTHRALATHR
12   TYRSERPROGLUPHETHRLYSVAL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks