BMRB Entry 34608

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PDB ID: 7nqc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34608
MolProbity Validation Chart

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Title:
Calmodulin extracts the Ras family protein RalA from lipid bilayers by engagement with two membrane targeting motifs
Deposition date:
2021-03-01
Original release date:
2021-09-17
Authors:
Chamberlain, S.; Owen, D.; Mott, H.
Citation:

Citation: Chamberlain, S.; Gohlke, A.; Shafiq, A.; Squires, I.; Owen, D.; Mott, H.. "Calmodulin extracts the Ras family protein RalA from lipid bilayers by engagement with two membrane-targeting motifs"  Proc. Natl. Acad. Sci. U. S. A. 118, e2104219118-e2104219118 (2021).
PubMed: 34480001

Assembly members:

Assembly members:
entity_1, polymer, 165 residues, 18572.418 Da.
entity_2, polymer, 18 residues, 2177.687 Da.
entity_CA, non-polymer, 40.078 Da.
entity_ULW, non-polymer, 377.518 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pQE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRGSHHHHHHGSMADQLTEE QIAEFKEAFSLFDKDGDGTI TTKELGTVMRSLGQNPTEAE LQDMINEVDADGNGTIDFPE FLTMMARKMKDTDSEEEIRE AFRVFDKDGNGYISAAELRH VMTNLGEKLTDEEVDEMIRE ADIDGDGQVNYEEFVQMMTA KGGSL
entity_2: PNGKKKRKSLAKRIRERC

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts140
1H chemical shifts1087

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_33
4unit_43
5unit_53
6unit_63
7unit_74

Entities:

Entity 1, unit_1 165 residues - 18572.418 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERMETALAASPGLNLEUTHRGLUGLU
3   GLNILEALAGLUPHELYSGLUALAPHESER
4   LEUPHEASPLYSASPGLYASPGLYTHRILE
5   THRTHRLYSGLULEUGLYTHRVALMETARG
6   SERLEUGLYGLNASNPROTHRGLUALAGLU
7   LEUGLNASPMETILEASNGLUVALASPALA
8   ASPGLYASNGLYTHRILEASPPHEPROGLU
9   PHELEUTHRMETMETALAARGLYSMETLYS
10   ASPTHRASPSERGLUGLUGLUILEARGGLU
11   ALAPHEARGVALPHEASPLYSASPGLYASN
12   GLYTYRILESERALAALAGLULEUARGHIS
13   VALMETTHRASNLEUGLYGLULYSLEUTHR
14   ASPGLUGLUVALASPGLUMETILEARGGLU
15   ALAASPILEASPGLYASPGLYGLNVALASN
16   TYRGLUGLUPHEVALGLNMETMETTHRALA
17   LYSGLYGLYSERLEU

Entity 2, unit_2 18 residues - 2177.687 Da.

1   PROASNGLYLYSLYSLYSARGLYSSERLEU
2   ALALYSARGILEARGGLUARGCYS

Entity 3, unit_3 - Ca - 40.078 Da.

1   CA

Entity 4, unit_7 - 377.518 Da.

1   ULW

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