BMRB Entry 34645
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Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34645
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Title: Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles
Deposition date: 2021-07-08 Original release date: 2023-01-10
Authors: Wang, X.; Bouaziz, S.
Citation: Wang, X.. "Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles" .
Assembly members:
entity_1, polymer, 13 residues, 1536.725 Da.
Natural source: Common Name: SIV-mac, Simian immunodeficiency virus rhesus monkey Taxonomy ID: 11735 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus SIV
Experimental source: Production method: recombinant technology Host organism: Synthecium
Entity Sequences (FASTA):
entity_1: EDPAVDLLKNYMQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 13 residues - 1536.725 Da.
| 1 | GLU | ASP | PRO | ALA | VAL | ASP | LEU | LEU | LYS | ASN | ||||
| 2 | TYR | MET | GLN |
Samples:
sample_1: SIVmac p6 1.0 mM
sample_conditions_1: ionic strength: 50 mM; pH: 4.0; pressure: 1 atm; temperature: 323 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TopSpin, Bruker Biospin - processing
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AVANCE 600 MHz