BMRB Entry 34647
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34647
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Title: Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles
Deposition date: 2021-07-08 Original release date: 2023-01-10
Authors: Wang, X.; Bouaziz, S.
Citation: Wang, X.; Bouaziz, S.. "Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles" .
Assembly members:
entity_1, polymer, 63 residues, 7242.185 Da.
Natural source: Common Name: SIV Taxonomy ID: 11723 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus SIV
Experimental source: Production method: recombinant technology Host organism: Synthecium
Entity Sequences (FASTA):
entity_1: PMAQVHQGLMPTAPPEDPAV
DLLKNYMQLGKQQREKQRES
REKPYKEVTEDLLHLNSLFG
GDQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 23 |
| 15N chemical shifts | 31 |
| 1H chemical shifts | 305 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 63 residues - 7242.185 Da.
| 1 | PRO | MET | ALA | GLN | VAL | HIS | GLN | GLY | LEU | MET | ||||
| 2 | PRO | THR | ALA | PRO | PRO | GLU | ASP | PRO | ALA | VAL | ||||
| 3 | ASP | LEU | LEU | LYS | ASN | TYR | MET | GLN | LEU | GLY | ||||
| 4 | LYS | GLN | GLN | ARG | GLU | LYS | GLN | ARG | GLU | SER | ||||
| 5 | ARG | GLU | LYS | PRO | TYR | LYS | GLU | VAL | THR | GLU | ||||
| 6 | ASP | LEU | LEU | HIS | LEU | ASN | SER | LEU | PHE | GLY | ||||
| 7 | GLY | ASP | GLN |
Samples:
sample_1: SIVmac p6 1 mM
sample_conditions_1: ionic strength: 50 mM; pH: 4; pressure: 1 atm; temperature: 323 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TopSpin, Bruker Biospin - processing
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis, CCPN - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AVANCE 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts