BMRB

Biological Magnetic Resonance Data Bank


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BMRB Entry 34729

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34729
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Title: The NMR structure of the MAX67 effector from Magnaporthe Oryzae   PubMed: 35657473

Deposition date: 2022-04-12 Original release date: 2023-04-18

Authors: Lahfa, M.; Padilla, A.; de Guillen, K.; Pissarra, J.; Raji, M.; Cesari, S.; Kroj, T.; Gladieux, P.; Roumestand, C.; Barthe, P.

Citation: Lahfa, M.; Padilla, A.; de Guillen, K.; Pissarra, J.; Raji, M.; Cesari, S.; Kroj, T.; Gladieux, P.; Roumestand, C.; Barthe, P.. "1H, 13C, 15N backbone and side-chain NMR assignments for three MAX effectors from Magnaporthe oryzae"  Biomol. NMR Assign. 16, 305-309 (2022).

Assembly members:
entity_1, polymer, 60 residues, 6717.538 Da.

Natural source:   Common Name: rice blast fungus   Taxonomy ID: 318829   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Pyricularia oryzae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):
entity_1: GPHMADCTLGCKYLENNRWV SVSKSANIGDTLYIMGHSTK IGRGCKPETTEWSDAEIYSW

Data sets:
Data typeCount
13C chemical shifts253
15N chemical shifts65
1H chemical shifts405

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 60 residues - 6717.538 Da.

1   GLYPROHISMETALAASPCYSTHRLEUGLY
2   CYSLYSTYRLEUGLUASNASNARGTRPVAL
3   SERVALSERLYSSERALAASNILEGLYASP
4   THRLEUTYRILEMETGLYHISSERTHRLYS
5   ILEGLYARGGLYCYSLYSPROGLUTHRTHR
6   GLUTRPSERASPALAGLUILETYRSERTRP

Samples:

sample_1: MAX67, [U-15N], 0.5 mM; sodium acetate 25 mM; DTT 2 mM

sample_2: MAX67, [U-13C; U-15N], 0.5 mM; sodium acetate 25 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0 mM; pH: 4.6; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1

Software:

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation

CINDY v2.1, Padilla, A. - chemical shift assignment

TopSpin v3.6, Bruker Biospin - peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts