BMRB Entry 36136

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PDB ID: 5yxi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR36136
MolProbity Validation Chart

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Title:
Designed protein dRafX6
Deposition date:
2017-12-05
Original release date:
2018-12-03
Authors:
Liu, R.
Citation:

Citation: Liu, Ruicun; Wang, Jichao; Xiong, Peng; Chen, Quan; Liu, Haiyan. "De novo sequence redesign of a functional Ras-binding domain globally inverted the surface charge distribution and led to extreme thermostability"  Biotechnol. Bioeng. 118, 2031-2042 (2021).
PubMed: 33590881

Assembly members:

Assembly members:
dRafX6, polymer, 79 residues, 8763.368 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dRafX6: MADRTIEVELPNKQRTVINV RPGLTLKEALKKALKVRGID PNKVQVYLLLSGDDGAEQPL SLNHPAERLIGKKLKVVPL

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts69
1H chemical shifts550

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 79 residues - 8763.368 Da.

1   METALAASPARGTHRILEGLUVALGLULEU
2   PROASNLYSGLNARGTHRVALILEASNVAL
3   ARGPROGLYLEUTHRLEULYSGLUALALEU
4   LYSLYSALALEULYSVALARGGLYILEASP
5   PROASNLYSVALGLNVALTYRLEULEULEU
6   SERGLYASPASPGLYALAGLUGLNPROLEU
7   SERLEUASNHISPROALAGLUARGLEUILE
8   GLYLYSLYSLEULYSVALVALPROLEU

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