BMRB Entry 4767
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4767
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Title: 1Ha and 13Ca, 13Cb shifts of apocytochrome b562 at pH 5.1 (temperature 293 K) PubMed: 11969411
Deposition date: 2000-06-20 Original release date: 2002-09-23
Authors: D'Amelio, Nicola; Bonvin, Alexandre; Czisch, Michael; Barker, Paul; Kaptein, Rob
Citation: D'Amelio, Nicola; Bonvin, Alexandre; Czisch, Michael; Barker, Paul; Kaptein, Rob. "The C terminus of apocytochrome b562 undergoes fast motions and slow exchange among ordered conformations resembling the folded state" Biochemistry 41, 5505-5514 (2002).
Assembly members:
apocytochrome b562, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
apocytochrome b562: ADLEDNMETLNDNLKVIEKA
DNAAQVKDALTKMRAAALDA
QKATPPKLEDKSPDSPEMKD
FRHGFDILVGQIDDALKLAN
EGKVKEAQAAAEQLKTTRNA
YHQKYR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 108 |
| 13C chemical shifts | 303 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apo b562 | 1 |
Entities:
Entity 1, apo b562 106 residues - Formula weight is not available
| 1 | ALA | ASP | LEU | GLU | ASP | ASN | MET | GLU | THR | LEU | ||||
| 2 | ASN | ASP | ASN | LEU | LYS | VAL | ILE | GLU | LYS | ALA | ||||
| 3 | ASP | ASN | ALA | ALA | GLN | VAL | LYS | ASP | ALA | LEU | ||||
| 4 | THR | LYS | MET | ARG | ALA | ALA | ALA | LEU | ASP | ALA | ||||
| 5 | GLN | LYS | ALA | THR | PRO | PRO | LYS | LEU | GLU | ASP | ||||
| 6 | LYS | SER | PRO | ASP | SER | PRO | GLU | MET | LYS | ASP | ||||
| 7 | PHE | ARG | HIS | GLY | PHE | ASP | ILE | LEU | VAL | GLY | ||||
| 8 | GLN | ILE | ASP | ASP | ALA | LEU | LYS | LEU | ALA | ASN | ||||
| 9 | GLU | GLY | LYS | VAL | LYS | GLU | ALA | GLN | ALA | ALA | ||||
| 10 | ALA | GLU | GLN | LEU | LYS | THR | THR | ARG | ASN | ALA | ||||
| 11 | TYR | HIS | GLN | LYS | TYR | ARG |
Samples:
sample_1: apocytochrome b562, [U-15N; U-13C], 1 mM
condition_1: pH: 5.1; temperature: 293 K; ionic strength: 0.2 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCO | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
Software:
REGINE -
NMR spectrometers:
- Varian Inova 500 MHz
Related Database Links:
| BMRB | 1672 4759 |
| PDB | |
| DBJ | BAB38636 BAG80065 BAI28542 BAI33713 BAI38842 |
| EMBL | CAA47706 CAP78754 CAQ34585 CAQ91733 CAR01210 |
| GB | AAA97133 AAB20782 AAG59433 AAN45672 AAN83756 |
| PIR | E86121 E91280 |
| REF | NP_290867 NP_313240 NP_709965 NP_757182 WP_000112424 |
| SP | P0ABE7 P0ABE8 Q8CVG7 |
| AlphaFold | P0ABE7 P0ABE8 Q8CVG7 |