BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4906

Title: Solution Structure of a C-Terminal Coiled-Coil Domain from Bovine IF1 - the Inhibitor Protein of F1 ATPase

Deposition date: 2000-11-30 Original release date: 2001-05-09

Authors: Gordon-Smith, Duncan; Carbajo, Rodrigo; Yang, Ji-Chung; Hortense, Videler; Runswick, Michael; Walker, John; Neuhaus, David

Citation: Gordon-Smith, Duncan; Carbajo, Rodrigo; Yang, Ji-Chun; Videler, Hortense; Runswick, Michael; Walker, John; Neuhaus, David. "Solution Structure of a C-terminal Coiled-coil Domain from Bovine IF1: The Inhibitor Protein of F1 ATPase"  J. Mol. Biol. 308, 325-339 (2001).

Assembly members:
Inhibitor of F1 ATPase, polymer, 41 residues, 4984 Da.

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMW172

Entity Sequences (FASTA):
Inhibitor of F1 ATPase: ALKKHHENEISHHAKEIERL QKEIERHKQSIKKLKQSEDD D

Data sets:
Data typeCount
1H chemical shifts306
13C chemical shifts180
15N chemical shifts54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IF1 44-84 subunit 11
2IF1 44-84 subunit 21

Entities:

Entity 1, IF1 44-84 subunit 1 41 residues - 4984 Da.

1   ALALEULYSLYSHISHISGLUASNGLUILE
2   SERHISHISALALYSGLUILEGLUARGLEU
3   GLNLYSGLUILEGLUARGHISLYSGLNSER
4   ILELYSLYSLEULYSGLNSERGLUASPASP
5   ASP

Samples:

sample_1: Inhibitor of F1 ATPase, [U-13C; U-15N], 1.5 mM

Ex-cond_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-1H COSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
15N NOESY HMQCnot availablenot availablenot available
15N HMQC NOESY HMQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
13C HCCH TOCSYnot availablenot availablenot available
13C HSQC NOESYnot availablenot availablenot available
13C,15N double half filtered NOESYnot availablenot availablenot available
13C edit/purge HSQC NOESYnot availablenot availablenot available

Software:

FELIX v97, Byosim Technologies - data analysis

X-WINNMR v2.1, Bruker - acquisition of spectra, processing of spectra

X-PLOR v3.1 - Structure calculations

NMR spectrometers:

  • Bruker DRX 300 MHz
  • Bruker AMX 500 MHz
  • Bruker DRX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 800 MHz

Related Database Links:

PDB
GB AAA30396 AAI11646 ELR56311
PRF 1009211A
REF NP_787010 XP_004023375 XP_005677044 XP_005895981 XP_005960342
SP P01096
TPG DAA32068
AlphaFold P01096

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts