BMRB Entry 50168
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50168
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: J-domain region of DnaJA2 PubMed: 33177718
Deposition date: 2020-01-26 Original release date: 2020-11-11
Authors: Rosenzweig, Rina; Abayev-Avraham, Meital
Citation: Rosenzweig, Rina; Abayev-Avraham, Maital; Faust, Ofrah; Maurer, Michael; Wentink, Anne; Bukau, Bernd; Nillegoda, Nadinath; London, Nir. "Hsp40s employ class-specific regulation to drive Hsp70 functional diversity" Nature 587, 489-494 (2020).
Assembly members:
J-domain of DnaJA2, polymer, 77 residues, 8777 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29b
Entity Sequences (FASTA):
J-domain of DnaJA2: MANVADTKLYDILGVPPGAS
ENELKKAYRKLAKEYHPDKN
PNAGDKFKEISFAYEVLSNP
EKRELYDRYGEQGLREG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 204 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | J-domain region of DnaJA2 | 1 |
Entities:
Entity 1, J-domain region of DnaJA2 77 residues - 8777 Da.
| 1 | MET | ALA | ASN | VAL | ALA | ASP | THR | LYS | LEU | TYR | ||||
| 2 | ASP | ILE | LEU | GLY | VAL | PRO | PRO | GLY | ALA | SER | ||||
| 3 | GLU | ASN | GLU | LEU | LYS | LYS | ALA | TYR | ARG | LYS | ||||
| 4 | LEU | ALA | LYS | GLU | TYR | HIS | PRO | ASP | LYS | ASN | ||||
| 5 | PRO | ASN | ALA | GLY | ASP | LYS | PHE | LYS | GLU | ILE | ||||
| 6 | SER | PHE | ALA | TYR | GLU | VAL | LEU | SER | ASN | PRO | ||||
| 7 | GLU | LYS | ARG | GLU | LEU | TYR | ASP | ARG | TYR | GLY | ||||
| 8 | GLU | GLN | GLY | LEU | ARG | GLU | GLY |
Samples:
sample_1: HEPES 50 mM; KCl 50 mM; sodium azide 0.03%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.0.6, Bruker Biospin - collection, processing
CcpNMR v2.4.2, CCPN - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts