BMRB Entry 50208
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50208
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Title: Purification of native CCL7 and its functional interaction with selected chemokine receptors PubMed: 32145391
Deposition date: 2020-03-07 Original release date: 2020-08-25
Authors: Goncharuk, Marina; Roy, Debarati; Dubinnyi, Maxim; Nadezhdin, Kirill; Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Arseniev, Alexander; Shukla, Arun
Citation: Goncharuk, Marina; Roy, Debarati; Dubinnyi, Maxim; Nadezhdin, Kirill; Srivastava, Ashish; Baidya, Mithu; Dwivedi-Agnihotri, Hemlata; Arseniev, Alexander; Shukla, Arun. "Purification of native CCL7 and its functional interaction with selected chemokine receptors" Protein Expr. Purif. 171, 105617-105617 (2020).
Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEMEX-1(his6)
Entity Sequences (FASTA):
entity_1: MHHHHHHGSQPVGINTSTTC
CYRFINKKIPKQRLESYRRT
TSSHCPREAVIFKTKLDKEI
CADPTQKWVQDFMKHLDKKT
QTPKL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 536 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HisCCL7 | 1 |
Entities:
Entity 1, HisCCL7 85 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | GLY | SER | GLN | ||||
| 2 | PRO | VAL | GLY | ILE | ASN | THR | SER | THR | THR | CYS | ||||
| 3 | CYS | TYR | ARG | PHE | ILE | ASN | LYS | LYS | ILE | PRO | ||||
| 4 | LYS | GLN | ARG | LEU | GLU | SER | TYR | ARG | ARG | THR | ||||
| 5 | THR | SER | SER | HIS | CYS | PRO | ARG | GLU | ALA | VAL | ||||
| 6 | ILE | PHE | LYS | THR | LYS | LEU | ASP | LYS | GLU | ILE | ||||
| 7 | CYS | ALA | ASP | PRO | THR | GLN | LYS | TRP | VAL | GLN | ||||
| 8 | ASP | PHE | MET | LYS | HIS | LEU | ASP | LYS | LYS | THR | ||||
| 9 | GLN | THR | PRO | LYS | LEU |
Samples:
sample_1: His6-tagged human chemokine CCL7, [U-100% 15N], 0.8 mM; KPi 20 mM; MES 0.4 mM; NaCl 7 mM
sample_conditions_1: ionic strength: 0.007 M; pH: 5.1; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.0 - collection
CARA v1.9.1.7 - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts