BMRB Entry 50313

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50313

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Title:
13C and 15N Chemical Shift Assignments for human M129V variant Y145Stop Prion Protein Amyloid Fibrils
Deposition date:
2020-06-10
Original release date:
2021-07-27
Authors:
Dao, Hanh; Hlaing, May; Ma, Yixuan; Surewicz, Krystyna; Surewicz, Witold; Jaroniec, Christopher
Citation:

Citation: Dao, Hanh; Hlaing, May; Ma, Yixuan; Surewicz, Krystyna; Surewicz, Witold; Jaroniec, Christopher. "13C and 15N chemical shift assignments of A117V and M129V human Y145Stop prion protein amyloid fibrils"  Biomol. NMR Assignments 15, 45-51 (2021).
PubMed: 33123960

Assembly members:

Assembly members:
entity_1, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSETB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSDPKKRPKPGGWNTGGSRY PGQGSPGGNRYPPQGGGGWG QPHGGGWGQPHGGGWGQPHG GGWGQPHGGGWGQGGGTHSQ WNKPSKPKTNMKHMAGAAAA GAVVGGLGGYVLGSAMSRPI IHFGSD

Data sets:
Data typeCount
13C chemical shifts62
15N chemical shifts18

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1huPrP23-144 M129V amyloid fibrils1

Entities:

Entity 1, huPrP23-144 M129V amyloid fibrils 126 residues - Formula weight is not available

The four N-terminal residues (GSDP) correspond to non-native thrombin cleavage sites used for protein purification. This is a truncated version of the human prion protein. This Y145Stop human prion protein includes residues 23 to 144, starting at 23K, 24K, 25R, and ending at 142G, 143S, 144D.

1   GLYSERASPPROLYSLYSARGPROLYSPRO
2   GLYGLYTRPASNTHRGLYGLYSERARGTYR
3   PROGLYGLNGLYSERPROGLYGLYASNARG
4   TYRPROPROGLNGLYGLYGLYGLYTRPGLY
5   GLNPROHISGLYGLYGLYTRPGLYGLNPRO
6   HISGLYGLYGLYTRPGLYGLNPROHISGLY
7   GLYGLYTRPGLYGLNPROHISGLYGLYGLY
8   TRPGLYGLNGLYGLYGLYTHRHISSERGLN
9   TRPASNLYSPROSERLYSPROLYSTHRASN
10   METLYSHISMETALAGLYALAALAALAALA
11   GLYALAVALVALGLYGLYLEUGLYGLYTYR
12   VALLEUGLYSERALAMETSERARGPROILE
13   ILEHISPHEGLYSERASP