BMRB Entry 50366
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50366
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Title: hCEACAM1 N-terminal IgV domain N97A mutant PubMed: 33742094
Deposition date: 2020-06-26 Original release date: 2021-03-22
Authors: Sun, Zhen-Yu; Wagner, Gerhard; Kim, Walter; Huang, Yu-Hwa; Blumberg, Richard; Gandhi, Amit
Citation: Gandhi, Amit; Sun, Zhen-Yu; Kim, Walter; Huang, Yu-Hwa; Kondo, Yasuyuki; Bonsor, Daniel; Sundberg, Eric; Kuchroo, Vijay; Petsko, Gregory; Blumberg, Richard. "Structural basis of the dynamic human CEACAM1 monomer-dimer equilibrium" Commun. Biol. 4, 360-360 (2021).
Assembly members:
entity_1, polymer, 108 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d
Entity Sequences (FASTA):
entity_1: AQLTTESMPFNVAEGKEVLL
LVHNLPQQLFGYSWYKGERV
DGNRQIVGYAIGTQQATPGP
ANSGRETIYPNASLLIQNVT
QNDTGFYTLQVIKSDLVAEE
ATGQFHVY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 92 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hCEACAM1 N-terminal IgV domain N97A mutant | 1 |
Entities:
Entity 1, hCEACAM1 N-terminal IgV domain N97A mutant 108 residues - Formula weight is not available
| 1 | ALA | GLN | LEU | THR | THR | GLU | SER | MET | PRO | PHE | ||||
| 2 | ASN | VAL | ALA | GLU | GLY | LYS | GLU | VAL | LEU | LEU | ||||
| 3 | LEU | VAL | HIS | ASN | LEU | PRO | GLN | GLN | LEU | PHE | ||||
| 4 | GLY | TYR | SER | TRP | TYR | LYS | GLY | GLU | ARG | VAL | ||||
| 5 | ASP | GLY | ASN | ARG | GLN | ILE | VAL | GLY | TYR | ALA | ||||
| 6 | ILE | GLY | THR | GLN | GLN | ALA | THR | PRO | GLY | PRO | ||||
| 7 | ALA | ASN | SER | GLY | ARG | GLU | THR | ILE | TYR | PRO | ||||
| 8 | ASN | ALA | SER | LEU | LEU | ILE | GLN | ASN | VAL | THR | ||||
| 9 | GLN | ASN | ASP | THR | GLY | PHE | TYR | THR | LEU | GLN | ||||
| 10 | VAL | ILE | LYS | SER | ASP | LEU | VAL | ALA | GLU | GLU | ||||
| 11 | ALA | THR | GLY | GLN | PHE | HIS | VAL | TYR |
Samples:
sample_1: hCEACAM1-N97A, [U-100% 13C; U-100% 15N], 0.3 mM; HEPES 10 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRj - collection
NMRPipe - processing
hmsIST - processing
CARA - chemical shift assignment
NMR spectrometers:
- Agilent DD2 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts