BMRB Entry 50427
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50427
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Title: FOXP2_247-341 PubMed: 33284517
Deposition date: 2020-08-06 Original release date: 2021-06-30
Authors: Richter, Gesa; Madl, Tobias
Citation: Richter, Gesa; Gui, Tianshu; Bourgeois, Benjamin; Koyani, Chintan; Ulz, Peter; Heitzer, Ellen; von Lewinski, Dirk; Burgering, Boudewijn; Malle, Ernst; Madl, Tobias. "b-catenin regulates FOXP2 transcriptional activity via multiple binding sites" FEBS J. 288, 3261-3284 (2021).
Assembly members:
entity_1, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petZ2_1a
Entity Sequences (FASTA):
entity_1: GQAALPVQSLPQAGLSPAEI
QQLWKEVTGVHSMEDNGIKH
GGLDLTTNNSSSTTSSNTSK
ASPPITHHSIVNGQSSVLSA
RRDSSSHEETGASHT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 180 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FOXP2_247-341 | 1 |
Entities:
Entity 1, FOXP2_247-341 95 residues - Formula weight is not available
| 1 | GLY | GLN | ALA | ALA | LEU | PRO | VAL | GLN | SER | LEU | ||||
| 2 | PRO | GLN | ALA | GLY | LEU | SER | PRO | ALA | GLU | ILE | ||||
| 3 | GLN | GLN | LEU | TRP | LYS | GLU | VAL | THR | GLY | VAL | ||||
| 4 | HIS | SER | MET | GLU | ASP | ASN | GLY | ILE | LYS | HIS | ||||
| 5 | GLY | GLY | LEU | ASP | LEU | THR | THR | ASN | ASN | SER | ||||
| 6 | SER | SER | THR | THR | SER | SER | ASN | THR | SER | LYS | ||||
| 7 | ALA | SER | PRO | PRO | ILE | THR | HIS | HIS | SER | ILE | ||||
| 8 | VAL | ASN | GLY | GLN | SER | SER | VAL | LEU | SER | ALA | ||||
| 9 | ARG | ARG | ASP | SER | SER | SER | HIS | GLU | GLU | THR | ||||
| 10 | GLY | ALA | SER | HIS | THR |
Samples:
sample_1: FOXP2_247-341, [U-99% 13C; U-99% 15N], 500 uM; sodium chloride 150 mM; sodium phosphate 50 mM; beta-mercaptoethanol 2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)NNH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
TOPSPIN - collection, processing
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts