BMRB Entry 50507
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50507
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NMR-STAR v3 text file.
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Title: 1H, 15N and 13C NMR assignments of the N-terminal domain of HKU1-bCoV nucleoprotein. PubMed: 33389548
Deposition date: 2020-10-10 Original release date: 2021-10-01
Authors: Caruso, Icaro; Marques, Aline; Santana-Silva, Marcos; Araujo, Gabriela; Bezerra, Peter; Almeida, Fabio; Amorim, Gisele
Citation: de Luna Marques, Aline; Caruso, Icaro Putinhon; Santana-Silva, Marcos Caique; Bezerra, Peter Reis; Araujo, Gabriela Rocha; Almeida, Fabio Ceneviva Lacerda; Amorim, Gisele Cardoso. "1H,15N and 13C resonance assignments of the N-terminal domain of the nucleocapsid protein from the endemic human coronavirus HKU1" Biomol. NMR Assign. 15, 153-157 (2021).
Assembly members:
HKU1-CoV nucleoprotein N-terminal domain, polymer, 138 residues, Formula weight is not available
Natural source: Common Name: HKU1 betacoronavirus Taxonomy ID: 694002 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HKU1 betacoronavirus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
HKU1-CoV nucleoprotein N-terminal domain: GNTIPHYSWFSGITQFQKGR
DFKFSDGQGVPIAFGVPPSE
AKGYWYRHSRRSFKTADGQQ
KQLLPRWYFYYLGTGPYANA
SYGESLEGVFWVANHQADTS
TPSDVSSRDPTTQEAIPTRF
PPGTILPQGYYVEGSGRS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 561 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 875 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-NTD | 1 |
Entities:
Entity 1, N-NTD 138 residues - Formula weight is not available
| 1 | GLY | ASN | THR | ILE | PRO | HIS | TYR | SER | TRP | PHE | ||||
| 2 | SER | GLY | ILE | THR | GLN | PHE | GLN | LYS | GLY | ARG | ||||
| 3 | ASP | PHE | LYS | PHE | SER | ASP | GLY | GLN | GLY | VAL | ||||
| 4 | PRO | ILE | ALA | PHE | GLY | VAL | PRO | PRO | SER | GLU | ||||
| 5 | ALA | LYS | GLY | TYR | TRP | TYR | ARG | HIS | SER | ARG | ||||
| 6 | ARG | SER | PHE | LYS | THR | ALA | ASP | GLY | GLN | GLN | ||||
| 7 | LYS | GLN | LEU | LEU | PRO | ARG | TRP | TYR | PHE | TYR | ||||
| 8 | TYR | LEU | GLY | THR | GLY | PRO | TYR | ALA | ASN | ALA | ||||
| 9 | SER | TYR | GLY | GLU | SER | LEU | GLU | GLY | VAL | PHE | ||||
| 10 | TRP | VAL | ALA | ASN | HIS | GLN | ALA | ASP | THR | SER | ||||
| 11 | THR | PRO | SER | ASP | VAL | SER | SER | ARG | ASP | PRO | ||||
| 12 | THR | THR | GLN | GLU | ALA | ILE | PRO | THR | ARG | PHE | ||||
| 13 | PRO | PRO | GLY | THR | ILE | LEU | PRO | GLN | GLY | TYR | ||||
| 14 | TYR | VAL | GLU | GLY | SER | GLY | ARG | SER |
Samples:
sample_1: N-NTD, [U-98% 13C; U-98% 15N], 250 uM; sodium phosphate 20 mM; PMSF 0.5 mM; sodium chloride 50 mM; sodium azide 3 mM; EDTA 3 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 2D HBCBCGCDHD | sample_1 | isotropic | sample_conditions_1 |
| 2D HBCBCGCDCEHE | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6 - collection
NMRPipe v2020.084.12.02 - processing
NMRDraw v10.8 Rev 2020.072.12.11 - data analysis, processing
ANALYSIS v2.5.0 - chemical shift assignment, data analysis, peak picking, structure solution
ARIA2 v2.3.2 - refinement, structure solution
NMRbox v7.14 Rev 11801 - chemical shift assignment, data analysis, peak picking, processing, refinement, structure solution
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts