BMRB Entry 50541
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR50541
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Title: 13C and 15N Chemical Shift Assignments for the Tubular Assembly of the Rous Sarcoma Virus Capsid Protein PubMed: 28094514
Deposition date: 2020-10-29 Original release date: 2020-11-05
Authors: Jeon, Jaekyun; Qiao, Xin; Hung, Ivan; Mitra, Alok; Desfosses, Ambroise; Huang, Daniel; Gor'kov, Peter; Craven, Rebecca; Kingston, Richard; Gan, Zhehong; Zhu, Fangqiang; Chen, Bo
Citation: Jeon, Jaekyun; Qiao, Xin; Hung, Ivan; Mitra, Alok; Desfosses, Ambroise; Huang, Daniel; Gor'kov, Peter; Craven, Rebecca; Kingston, Richard; Gan, Zhehong; Zhu, Fangqiang; Chen, Bo. "Structural Model of the Tubular Assembly of the Rous Sarcoma Virus Capsid Protein." J. Am. Chem. Soc. 139, 2006-2013 (2017).
Assembly members:
entity_1, polymer, 237 residues, 25550 Da.
Natural source: Common Name: Rous sarcoma virus Taxonomy ID: 11886 Superkingdom: Viruses Kingdom: not available Genus/species: Alpharetrovirus Rous sarcoma virus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24(+)
Entity Sequences (FASTA):
entity_1: PVVIKTEGPAWTPLEPKLIT
RLADTVRTKGLRSPITMAEV
EALMSSPLLPHDVTNLMRVI
LGPAPYALWMDAWGVQLQTV
IAAATRDPRHPANGQGRGER
TNLNRLKGLADGMVGNPQGQ
AALLRPGELVAITASALQAF
REVARLAEPAGPWADIMQGP
SESFVDFANRLIKAVEGSDL
PPSARAPVIIDCFRQKSQPD
IQQLIRTAPSTLTTPGEIIK
YVLDRQKTAPLTDQGIA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1042 |
| 15N chemical shifts | 226 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rous Sarcoma Virus Capsid Protein, Monomer 1 | 1 |
| 2 | Rous Sarcoma Virus Capsid Protein, Monomer 2 | 1 |
| 3 | Rous Sarcoma Virus Capsid Protein, Monomer 3 | 1 |
| 4 | Rous Sarcoma Virus Capsid Protein, Monomer 4 | 1 |
| 5 | Rous Sarcoma Virus Capsid Protein, Monomer 5 | 1 |
| 6 | Rous Sarcoma Virus Capsid Protein, Monomer 6 | 1 |
Entities:
Entity 1, Rous Sarcoma Virus Capsid Protein, Monomer 1 237 residues - 25550 Da.
| 1 | PRO | VAL | VAL | ILE | LYS | THR | GLU | GLY | PRO | ALA | ||||
| 2 | TRP | THR | PRO | LEU | GLU | PRO | LYS | LEU | ILE | THR | ||||
| 3 | ARG | LEU | ALA | ASP | THR | VAL | ARG | THR | LYS | GLY | ||||
| 4 | LEU | ARG | SER | PRO | ILE | THR | MET | ALA | GLU | VAL | ||||
| 5 | GLU | ALA | LEU | MET | SER | SER | PRO | LEU | LEU | PRO | ||||
| 6 | HIS | ASP | VAL | THR | ASN | LEU | MET | ARG | VAL | ILE | ||||
| 7 | LEU | GLY | PRO | ALA | PRO | TYR | ALA | LEU | TRP | MET | ||||
| 8 | ASP | ALA | TRP | GLY | VAL | GLN | LEU | GLN | THR | VAL | ||||
| 9 | ILE | ALA | ALA | ALA | THR | ARG | ASP | PRO | ARG | HIS | ||||
| 10 | PRO | ALA | ASN | GLY | GLN | GLY | ARG | GLY | GLU | ARG | ||||
| 11 | THR | ASN | LEU | ASN | ARG | LEU | LYS | GLY | LEU | ALA | ||||
| 12 | ASP | GLY | MET | VAL | GLY | ASN | PRO | GLN | GLY | GLN | ||||
| 13 | ALA | ALA | LEU | LEU | ARG | PRO | GLY | GLU | LEU | VAL | ||||
| 14 | ALA | ILE | THR | ALA | SER | ALA | LEU | GLN | ALA | PHE | ||||
| 15 | ARG | GLU | VAL | ALA | ARG | LEU | ALA | GLU | PRO | ALA | ||||
| 16 | GLY | PRO | TRP | ALA | ASP | ILE | MET | GLN | GLY | PRO | ||||
| 17 | SER | GLU | SER | PHE | VAL | ASP | PHE | ALA | ASN | ARG | ||||
| 18 | LEU | ILE | LYS | ALA | VAL | GLU | GLY | SER | ASP | LEU | ||||
| 19 | PRO | PRO | SER | ALA | ARG | ALA | PRO | VAL | ILE | ILE | ||||
| 20 | ASP | CYS | PHE | ARG | GLN | LYS | SER | GLN | PRO | ASP | ||||
| 21 | ILE | GLN | GLN | LEU | ILE | ARG | THR | ALA | PRO | SER | ||||
| 22 | THR | LEU | THR | THR | PRO | GLY | GLU | ILE | ILE | LYS | ||||
| 23 | TYR | VAL | LEU | ASP | ARG | GLN | LYS | THR | ALA | PRO | ||||
| 24 | LEU | THR | ASP | GLN | GLY | ILE | ALA |
Samples:
sample_1: Capsid Protein, [U-100% 13C; U-100% 15N], 20 mg/mL; sodium phosphate 50 mM; polyethylene glycol 8000 20 w/v
sample_2: Capsid Protein, 1,3 13C Glycerol, 20 mg/mL; sodium phosphate 50 mM; polyethylene glycol 8000 20 w/v
sample_3: Capsid Protein, 2 13C Glycerol, 20 mg/mL; sodium phosphate 50 mM; polyethylene glycol 8000 20 w/v
sample_4: Capsid Protein, [U-13C; U-15N]-Leu, 20 mg/mL; sodium phosphate 50 mM; polyethylene glycol 8000 20 w/v
sample_5: Capsid Protein, [U-15N]-Leu, 20 mg/mL; sodium phosphate 50 mM; polyethylene glycol 8000 20 w/v
sample_conditions_1: pH: 8.0; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C DARR | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C/13C TOBSY | sample_1 | isotropic | sample_conditions_1 |
| 2D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 2D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D CaNCOCx | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-13C DARR | sample_2 | isotropic | sample_conditions_1 |
| 2D NCaCx | sample_2 | isotropic | sample_conditions_1 |
| 2D NCOCx | sample_2 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_2 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_2 | isotropic | sample_conditions_1 |
| 2D 13C-13C DARR | sample_3 | isotropic | sample_conditions_1 |
| 2D NCaCx | sample_3 | isotropic | sample_conditions_1 |
| 2D NCOCx | sample_3 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_3 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_3 | isotropic | sample_conditions_1 |
| 2D NCACX | sample_5 | isotropic | sample_conditions_1 |
| 2D NCOCX | sample_5 | isotropic | sample_conditions_1 |
| 2D NCACX | sample_4 | isotropic | sample_conditions_1 |
| 2D NCOCX | sample_4 | isotropic | sample_conditions_1 |
Software:
VNMRj v4.0 - collection
NMRPipe - processing
NMRFAM-SPARKY v1.470 - peak picking
mcassign2 - chemical shift assignment
NMR spectrometers:
- Agilent DDR2 600 MHz
- Brucker Avance 600 MHz
- Brucker Avance 900 MHz