BMRB Entry 50554
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50554
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Title: C8-AcpP FabF titration PubMed: 33727677
Deposition date: 2020-11-10 Original release date: 2021-05-18
Authors: Bartholow, Thomas
Citation: Bartholow, Thomas; Sztain, Terra; Patel, Ashay; Lee, D John; Young, Megan; Abagyan, Ruben; Burkart, Michael. "Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis" Commun. Biol. 4, 340-340 (2021).
Assembly members:
entity_1, polymer, 78 residues, Formula weight is not available
entity_2, polymer, 413 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28b
Entity Sequences (FASTA):
entity_1: MSTIEERVKKIIGEQLGVKQ
EEVTNNASFVEDLGADSLDT
VELVMALEEEFDTEIPDEEA
EKITTVQAAIDYINGHQA
entity_2: MSKRRVVVTGLGMLSPVGNT
VESTWKALLAGQSGISLIDH
FDTSAYATKFAGLVKDFNCE
DIISRKEQRKMDAFIQYGIV
AGVQAMQDSGLEITEENATR
IGAAIGSGIGGLGLIEENHT
SLMNGGPRKISPFFVPSTIV
NMVAGHLTIMYGLRGPSISI
ATACTSGVHNIGHAARIIAY
GDADVMVAGGAEKASTPLGV
GGFGAARALSTRNDNPQAAS
RPWDKERDGFVLGDGAGMLV
LEEYEHAKKRGAKIYAELVG
FGMSSDAYHMTSPPENGAGA
ALAMANALRDAGIEASQIGY
VNAHGTSTPAGDKAEAQAVK
TIFGEAASRVLVSSTKSMTG
HLLGAAGAVESIYSILALRD
QAVPPTINLDNPDEGCDLDF
VPHEARQVSGMEYTLCNSFG
FGGTNGSLIFKKI
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AcpP | 1 |
| 2 | FabF | 2 |
Entities:
Entity 1, AcpP 78 residues - Formula weight is not available
| 1 | MET | SER | THR | ILE | GLU | GLU | ARG | VAL | LYS | LYS | ||||
| 2 | ILE | ILE | GLY | GLU | GLN | LEU | GLY | VAL | LYS | GLN | ||||
| 3 | GLU | GLU | VAL | THR | ASN | ASN | ALA | SER | PHE | VAL | ||||
| 4 | GLU | ASP | LEU | GLY | ALA | ASP | SER | LEU | ASP | THR | ||||
| 5 | VAL | GLU | LEU | VAL | MET | ALA | LEU | GLU | GLU | GLU | ||||
| 6 | PHE | ASP | THR | GLU | ILE | PRO | ASP | GLU | GLU | ALA | ||||
| 7 | GLU | LYS | ILE | THR | THR | VAL | GLN | ALA | ALA | ILE | ||||
| 8 | ASP | TYR | ILE | ASN | GLY | HIS | GLN | ALA |
Entity 2, FabF 413 residues - Formula weight is not available
| 1 | MET | SER | LYS | ARG | ARG | VAL | VAL | VAL | THR | GLY | ||||
| 2 | LEU | GLY | MET | LEU | SER | PRO | VAL | GLY | ASN | THR | ||||
| 3 | VAL | GLU | SER | THR | TRP | LYS | ALA | LEU | LEU | ALA | ||||
| 4 | GLY | GLN | SER | GLY | ILE | SER | LEU | ILE | ASP | HIS | ||||
| 5 | PHE | ASP | THR | SER | ALA | TYR | ALA | THR | LYS | PHE | ||||
| 6 | ALA | GLY | LEU | VAL | LYS | ASP | PHE | ASN | CYS | GLU | ||||
| 7 | ASP | ILE | ILE | SER | ARG | LYS | GLU | GLN | ARG | LYS | ||||
| 8 | MET | ASP | ALA | PHE | ILE | GLN | TYR | GLY | ILE | VAL | ||||
| 9 | ALA | GLY | VAL | GLN | ALA | MET | GLN | ASP | SER | GLY | ||||
| 10 | LEU | GLU | ILE | THR | GLU | GLU | ASN | ALA | THR | ARG | ||||
| 11 | ILE | GLY | ALA | ALA | ILE | GLY | SER | GLY | ILE | GLY | ||||
| 12 | GLY | LEU | GLY | LEU | ILE | GLU | GLU | ASN | HIS | THR | ||||
| 13 | SER | LEU | MET | ASN | GLY | GLY | PRO | ARG | LYS | ILE | ||||
| 14 | SER | PRO | PHE | PHE | VAL | PRO | SER | THR | ILE | VAL | ||||
| 15 | ASN | MET | VAL | ALA | GLY | HIS | LEU | THR | ILE | MET | ||||
| 16 | TYR | GLY | LEU | ARG | GLY | PRO | SER | ILE | SER | ILE | ||||
| 17 | ALA | THR | ALA | CYS | THR | SER | GLY | VAL | HIS | ASN | ||||
| 18 | ILE | GLY | HIS | ALA | ALA | ARG | ILE | ILE | ALA | TYR | ||||
| 19 | GLY | ASP | ALA | ASP | VAL | MET | VAL | ALA | GLY | GLY | ||||
| 20 | ALA | GLU | LYS | ALA | SER | THR | PRO | LEU | GLY | VAL | ||||
| 21 | GLY | GLY | PHE | GLY | ALA | ALA | ARG | ALA | LEU | SER | ||||
| 22 | THR | ARG | ASN | ASP | ASN | PRO | GLN | ALA | ALA | SER | ||||
| 23 | ARG | PRO | TRP | ASP | LYS | GLU | ARG | ASP | GLY | PHE | ||||
| 24 | VAL | LEU | GLY | ASP | GLY | ALA | GLY | MET | LEU | VAL | ||||
| 25 | LEU | GLU | GLU | TYR | GLU | HIS | ALA | LYS | LYS | ARG | ||||
| 26 | GLY | ALA | LYS | ILE | TYR | ALA | GLU | LEU | VAL | GLY | ||||
| 27 | PHE | GLY | MET | SER | SER | ASP | ALA | TYR | HIS | MET | ||||
| 28 | THR | SER | PRO | PRO | GLU | ASN | GLY | ALA | GLY | ALA | ||||
| 29 | ALA | LEU | ALA | MET | ALA | ASN | ALA | LEU | ARG | ASP | ||||
| 30 | ALA | GLY | ILE | GLU | ALA | SER | GLN | ILE | GLY | TYR | ||||
| 31 | VAL | ASN | ALA | HIS | GLY | THR | SER | THR | PRO | ALA | ||||
| 32 | GLY | ASP | LYS | ALA | GLU | ALA | GLN | ALA | VAL | LYS | ||||
| 33 | THR | ILE | PHE | GLY | GLU | ALA | ALA | SER | ARG | VAL | ||||
| 34 | LEU | VAL | SER | SER | THR | LYS | SER | MET | THR | GLY | ||||
| 35 | HIS | LEU | LEU | GLY | ALA | ALA | GLY | ALA | VAL | GLU | ||||
| 36 | SER | ILE | TYR | SER | ILE | LEU | ALA | LEU | ARG | ASP | ||||
| 37 | GLN | ALA | VAL | PRO | PRO | THR | ILE | ASN | LEU | ASP | ||||
| 38 | ASN | PRO | ASP | GLU | GLY | CYS | ASP | LEU | ASP | PHE | ||||
| 39 | VAL | PRO | HIS | GLU | ALA | ARG | GLN | VAL | SER | GLY | ||||
| 40 | MET | GLU | TYR | THR | LEU | CYS | ASN | SER | PHE | GLY | ||||
| 41 | PHE | GLY | GLY | THR | ASN | GLY | SER | LEU | ILE | PHE | ||||
| 42 | LYS | LYS | ILE |
Samples:
sample_1: E. coli Acyl Carrier Protein, [U-100% 15N; U~90% 2H], 0.042 uM; E. coli 3-oxoacyl-[acyl-carrier-protein] synthase 2 0.0837 uM; sodium azide 0.1%; TCEP 0.5 mM; potassium phosphate 10 mM
sample_2: E. coli Acyl Carrier Protein, [U-100% 15N; U~90% 2H], 0.042 uM; sodium azide 0.1%; TCEP 0.5 mM; potassium phosphate 10 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC AcpP FabF titration saturated | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC AcpP FabF titration zero point | sample_2 | isotropic | sample_conditions_1 |
| Prior assignment | sample_2 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMR Pipe - processing
SPARKY - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts