BMRB Entry 50619

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50619

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H 13C 15N assignment of IDR1-domain (1-47) of Nucleocapsid Protein of SARS-CoV 2 in the 1-248 construct
Deposition date:
2020-12-04
Original release date:
2021-03-11
Authors:
Schiavina, Marco; Pontoriero, Letizia; Felli, Isabella Caterina; Pierattelli, Roberta
Citation:

Citation: Schiavina, Marco; Pontoriero, Letizia; Uversky, Vladimir; Felli, Isabella Caterina; Pierattelli, Roberta. "The highly flexible, disordered regions of the SARS-CoV 2 Nucleocapsid protein within the 1-248 residue construct: sequence specific resonance assignments through NMR"  Biomol. NMR Assignments 15, 219-227 (2021).
PubMed: 33660218

Assembly members:

Assembly members:
entity_1, polymer, 248 residues, 26500 Da.

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALN TPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALA LLLLDRLNQLESKMSGKGQQ QQGQTVTK

Data sets:
Data typeCount
13C chemical shifts130
15N chemical shifts45
1H chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-arm1

Entities:

Entity 1, N-arm 248 residues - 26500 Da.

1   METSERASPASNGLYPROGLNASNGLNARG
2   ASNALAPROARGILETHRPHEGLYGLYPRO
3   SERASPSERTHRGLYSERASNGLNASNGLY
4   GLUARGSERGLYALAARGSERLYSGLNARG
5   ARGPROGLNGLYLEUPROASNASNTHRALA
6   SERTRPPHETHRALALEUTHRGLNHISGLY
7   LYSGLUASPLEULYSPHEPROARGGLYGLN
8   GLYVALPROILEASNTHRASNSERSERPRO
9   ASPASPGLNILEGLYTYRTYRARGARGALA
10   THRARGARGILEARGGLYGLYASPGLYLYS
11   METLYSASPLEUSERPROARGTRPTYRPHE
12   TYRTYRLEUGLYTHRGLYPROGLUALAGLY
13   LEUPROTYRGLYALAASNLYSASPGLYILE
14   ILETRPVALALATHRGLUGLYALALEUASN
15   THRPROLYSASPHISILEGLYTHRARGASN
16   PROALAASNASNALAALAILEVALLEUGLN
17   LEUPROGLNGLYTHRTHRLEUPROLYSGLY
18   PHETYRALAGLUGLYSERARGGLYGLYSER
19   GLNALASERSERARGSERSERSERARGSER
20   ARGASNSERSERARGASNSERTHRPROGLY
21   SERSERARGGLYTHRSERPROALAARGMET
22   ALAGLYASNGLYGLYASPALAALALEUALA
23   LEULEULEULEUASPARGLEUASNGLNLEU
24   GLUSERLYSMETSERGLYLYSGLYGLNGLN
25   GLNGLNGLYGLNTHRVALTHRLYS

Related Database Links:

UNP P0DTC9
AlphaFold P0DTC9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks