BMRB Entry 50718
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50718
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Citation: Weber, Daniel; Reddy, U Venkateswara; Wang, Songlin; Larsen, Erik; Gopinath, Tata; Gustavsson, Martin; Cornea, Razvan; Thomas, David; De Simone, Alfonso; Veglia, Gianluigi. "Structural basis for allosteric control of the SERCA-Phospholamban membrane complex by Ca 2+ and phosphorylation" Elife 10, e66226-e66226 (2021).
PubMed: 33978571
Assembly members:
entity_1, polymer, 53 residues, 6166 Da.
Natural source: Common Name: rabbit Taxonomy ID: 9986 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oryctolagus cuniculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal-c2x
Entity Sequences (FASTA):
entity_1: AMEKVQYLTRSAIRRASTIE
MPQQARQNLQNLFINFALIL
IFLLLIAIIVMLL
- assigned_chemical_shifts
- dipolar_couplings
| Data type | Count |
| 15N chemical shifts | 22 |
| dipolar coupling values | 22 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PLN | 1 |
Entities:
Entity 1, PLN 53 residues - 6166 Da.
Monomeric cysteine-null mutant (C36A/C41F/C46A). N-terminal Ala0 left over from cleavage of MBP fusion protein.
| 1 | ALA | MET | GLU | LYS | VAL | GLN | TYR | LEU | THR | ARG | ||||
| 2 | SER | ALA | ILE | ARG | ARG | ALA | SER | THR | ILE | GLU | ||||
| 3 | MET | PRO | GLN | GLN | ALA | ARG | GLN | ASN | LEU | GLN | ||||
| 4 | ASN | LEU | PHE | ILE | ASN | PHE | ALA | LEU | ILE | LEU | ||||
| 5 | ILE | PHE | LEU | LEU | LEU | ILE | ALA | ILE | ILE | VAL | ||||
| 6 | MET | LEU | LEU |