BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5073

Title: Solution structure of the monomeric variant of the chemokine MIP-1beta   PubMed: 11535053

Deposition date: 2001-07-09 Original release date: 2003-01-07

Authors: Kim, S.; Jao, S.; Laurence, J.; LiWang, P.

Citation: Kim, S.; Jao, S.; Laurence, J.; LiWang, P.. "Structural Comparison of Monomeric Variants of the Chemokine MIP-1beta having Differing Ability to bind the Receptor CCR5"  Biochemistry 40, 10782-10791 (2001).

Assembly members:
MIP-1 beta, polymer, 69 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
MIP-1 beta: APMGSDPPTACCASYTARKL PRNFVVDYYETSSLCSQPAV VFQTKRSKQVCADPSESWVQ EYVYDLELN

Data sets:
Data typeCount
13C chemical shifts190
15N chemical shifts62
1H chemical shifts376

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MIP-1-beta1

Entities:

Entity 1, MIP-1-beta 69 residues - Formula weight is not available

1   ALAPROMETGLYSERASPPROPROTHRALA
2   CYSCYSALASERTYRTHRALAARGLYSLEU
3   PROARGASNPHEVALVALASPTYRTYRGLU
4   THRSERSERLEUCYSSERGLNPROALAVAL
5   VALPHEGLNTHRLYSARGSERLYSGLNVAL
6   CYSALAASPPROSERGLUSERTRPVALGLN
7   GLUTYRVALTYRASPLEUGLULEUASN

Samples:

sample_1: MIP-1 beta, [U-15N; U-13C], 1 – 2 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 20 mM; pH: 2.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

NMRPipe - processing

CNS v1.0 - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA34291 CAA37723 CAG46916
GenBank AAA51576 AAA57256 AAI07434 AAX07292 AAX07305
REF NP_002975
SWISS-PROT P13236

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts