BMRB Entry 50804
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50804
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Title: 1H, 13C and 15N chemical shift assignments of RNA binding protein RBM3 PubMed: 34837346
Deposition date: 2021-03-07 Original release date: 2021-12-13
Authors: Boral, Soumendu; Roy, Sayantani; Basak, Aditya; Maiti, Snigdha; De, Soumya
Citation: Roy, Sayantani; Boral, Soumendu; Maiti, Snigdha; Kushwaha, Tushar; Basak, Aditya; Lee, Woonghee; Basak, Amit; Gholap, Shivajirao; Inampudi, Krishna; De, Soumya. "Structural and dynamic studies of the human RNA binding protein RBM3 reveals the molecular basis of its oligomerization and RNA recognition" FEBS J. 289, 2847-2864 (2021).
Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28(a)
Entity Sequences (FASTA):
entity_1: MSSEEGKLFVGGLNFNTDEQ
ALEDHFSSFGPISEVVVVKD
RETQRSRGFGFITFTNPEHA
SVAMRAMNGESLDGRQIRVD
HAGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 322 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 522 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA Recognition Motif | 1 |
Entities:
Entity 1, RNA Recognition Motif 84 residues - Formula weight is not available
| 1 | MET | SER | SER | GLU | GLU | GLY | LYS | LEU | PHE | VAL | ||||
| 2 | GLY | GLY | LEU | ASN | PHE | ASN | THR | ASP | GLU | GLN | ||||
| 3 | ALA | LEU | GLU | ASP | HIS | PHE | SER | SER | PHE | GLY | ||||
| 4 | PRO | ILE | SER | GLU | VAL | VAL | VAL | VAL | LYS | ASP | ||||
| 5 | ARG | GLU | THR | GLN | ARG | SER | ARG | GLY | PHE | GLY | ||||
| 6 | PHE | ILE | THR | PHE | THR | ASN | PRO | GLU | HIS | ALA | ||||
| 7 | SER | VAL | ALA | MET | ARG | ALA | MET | ASN | GLY | GLU | ||||
| 8 | SER | LEU | ASP | GLY | ARG | GLN | ILE | ARG | VAL | ASP | ||||
| 9 | HIS | ALA | GLY | LYS |
Samples:
sample_1: RNA Recognition Motif, [U-100% 15N], 1.0 mM; RNA Recognition Motif, [U-100% 13C; U-100% 15N], 0.6 mM; RNA Recognition Motif, [U-10% 13C; U-100% 15N], 0.7 mM; sodium phosphate buffer 10 mM; NaCl 200 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D (CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts