BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5091

Title: Solution structure of A67V mutant of rat ferro cytochrome B5

Deposition date: 2001-07-19 Original release date: 2003-06-02

Authors: Shahzad, N.; Dangi, B.; Blankman, J.; Guiles, R.

Citation: Shahzad, N.; Dangi, B.; Blankman, J.; Guiles, R.. "Mutagenic modulation of the entropy change on oxidation of cytochrome B5: an analysis of the contribution of conformational entropy "  .

Assembly members:
CYTOCHROME B5, polymer, 94 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CYTOCHROME B5: DKDVKYYTLEEIQKHKDSKS TWVILHHKVYDLTKFLEEHP GGEEVLREQAGGDATENFED VGHSTDVRELSKTYIIGELH PDDRSKIAKPSETL

Data sets:
Data typeCount
1H chemical shifts496
coupling constants66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYTOCHROME B51
2HEM2

Entities:

Entity 1, CYTOCHROME B5 94 residues - Formula weight is not available

1   ASPLYSASPVALLYSTYRTYRTHRLEUGLU
2   GLUILEGLNLYSHISLYSASPSERLYSSER
3   THRTRPVALILELEUHISHISLYSVALTYR
4   ASPLEUTHRLYSPHELEUGLUGLUHISPRO
5   GLYGLYGLUGLUVALLEUARGGLUGLNALA
6   GLYGLYASPALATHRGLUASNPHEGLUASP
7   VALGLYHISSERTHRASPVALARGGLULEU
8   SERLYSTHRTYRILEILEGLYGLULEUHIS
9   PROASPASPARGSERLYSILEALALYSPRO
10   SERGLUTHRLEU

Entity 2, HEM - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: CYTOCHROME B5 2 mM; phosphate buffer 1 mM; sodium dithionate mM; TSP 0.05 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7.0; temperature: 313 K; ionic strength: 1 mM

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1

Software:

DYANA v1.5 - refinement

XWINNMR v2.1 - collection

FELIX v97 - data analysis

NMRPipe v1.8 - processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

REF NP_071581
SWISS-PROT P00173
GenBank AAH86945 EDL75180 AAA72557 AAB67610 AAA72420
DBJ BAA02492
PDB
BMRB 4130 4131