BMRB Entry 50936
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50936
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Title: DksA N88D PubMed: 25799498
Deposition date: 2021-05-10 Original release date: 2021-06-10
Authors: Danhart, Eric
Citation: Furman, Ran; Danhart, Eric; NandyMazumdar, Monali; Yuan, Chunhua; Foster, Mark; Artsimovitch, Irina. "pH dependence of the stress regulator DksA." PLoS One 10, e0120746-e0120746 (2015).
Assembly members:
entity_1, polymer, 154 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pIA1119
Entity Sequences (FASTA):
entity_1: GLQMQEGQNRKTSSLSILAI
AGVEPYQEKPGEEYMNEAQL
AHFRRILEAWRNQLRDEVDR
TVTHMQDEAANFPDPVDRAA
QEEEFSLELRDRDRERKLIK
KIEKTLKKVEDEDFGYCESC
GVEIGIRRLEARPTADLCID
CKTLAEIREKQMAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 296 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 205 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DksA | 1 |
Entities:
Entity 1, DksA 154 residues - Formula weight is not available
| 1 | GLY | LEU | GLN | MET | GLN | GLU | GLY | GLN | ASN | ARG | ||||
| 2 | LYS | THR | SER | SER | LEU | SER | ILE | LEU | ALA | ILE | ||||
| 3 | ALA | GLY | VAL | GLU | PRO | TYR | GLN | GLU | LYS | PRO | ||||
| 4 | GLY | GLU | GLU | TYR | MET | ASN | GLU | ALA | GLN | LEU | ||||
| 5 | ALA | HIS | PHE | ARG | ARG | ILE | LEU | GLU | ALA | TRP | ||||
| 6 | ARG | ASN | GLN | LEU | ARG | ASP | GLU | VAL | ASP | ARG | ||||
| 7 | THR | VAL | THR | HIS | MET | GLN | ASP | GLU | ALA | ALA | ||||
| 8 | ASN | PHE | PRO | ASP | PRO | VAL | ASP | ARG | ALA | ALA | ||||
| 9 | GLN | GLU | GLU | GLU | PHE | SER | LEU | GLU | LEU | ARG | ||||
| 10 | ASP | ARG | ASP | ARG | GLU | ARG | LYS | LEU | ILE | LYS | ||||
| 11 | LYS | ILE | GLU | LYS | THR | LEU | LYS | LYS | VAL | GLU | ||||
| 12 | ASP | GLU | ASP | PHE | GLY | TYR | CYS | GLU | SER | CYS | ||||
| 13 | GLY | VAL | GLU | ILE | GLY | ILE | ARG | ARG | LEU | GLU | ||||
| 14 | ALA | ARG | PRO | THR | ALA | ASP | LEU | CYS | ILE | ASP | ||||
| 15 | CYS | LYS | THR | LEU | ALA | GLU | ILE | ARG | GLU | LYS | ||||
| 16 | GLN | MET | ALA | GLY |
Samples:
sample_1: DksA N88D, [U-13C; U-15N], 0.4 mM; NaCl 200 mM; DTT 1 mM; ZnCl2 50 uM; sodium phosphate 200 mM; sodium azide 0.02%
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 15N-(1H) NOE | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRViewJ - chemical shift assignment
TOPSPIN - collection
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts