BMRB Entry 51257
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51257
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Title: Denatured State Backbone Assignments for UBA(1) from HHR23A in Guanidine Hydrochloride Solutions PubMed: 35430812
Deposition date: 2022-01-04 Original release date: 2022-06-08
Authors: Becht, Dustin; Briknarova, Klara; Bowler, Bruce
Citation: Becht, Dustin; Leavens, Moses; Zeng, Baisen; Rothfuss, Michael; Briknarova, Klara; Bowler, Bruce. "Residual Structure in the Denatured State of the Fast-Folding UBA(1) Domain from the Human DNA Excision Repair Protein HHR23A" Biochemistry 61, 767-784 (2022).
Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T
Entity Sequences (FASTA):
entity_1: STLVTGSEYETMLTEIMSMG
YERERVVAALRASYNNPHRA
VEYLLTGIPG
Data type | Count |
13C chemical shifts | 388 |
15N chemical shifts | 188 |
1H chemical shifts | 188 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | UBA(1) | 1 |
Entities:
Entity 1, UBA(1) 50 residues - Formula weight is not available
1 | SER | THR | LEU | VAL | THR | GLY | SER | GLU | TYR | GLU | |
2 | THR | MET | LEU | THR | GLU | ILE | MET | SER | MET | GLY | |
3 | TYR | GLU | ARG | GLU | ARG | VAL | VAL | ALA | ALA | LEU | |
4 | ARG | ALA | SER | TYR | ASN | ASN | PRO | HIS | ARG | ALA | |
5 | VAL | GLU | TYR | LEU | LEU | THR | GLY | ILE | PRO | GLY |
Samples:
sample_1: UBA(1), [U-13C; U-15N], 0.625 mM; sodium phosphate 50 mM; NaCl 100 mM; guanidine hydrochloride 4 M
sample_2: UBA(1), [U-13C; U-15N], 0.625 mM; sodium phosphate 50 mM; NaCl 100 mM; guanidine hydrochloride 5 M
sample_3: UBA(1), [U-13C; U-15N], 0.625 mM; sodium phosphate 50 mM; NaCl 100 mM; guanidine hydrochloride 6 M
sample_4: UBA(1), [U-13C; U-15N], 0.625 mM; sodium phosphate 50 mM; NaCl 100 mM; guanidine hydrochloride 7 M
sample_conditions_1: ionic strength: 4 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 5 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
sample_conditions_3: ionic strength: 6 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
sample_conditions_4: ionic strength: 7 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
3D HNCO | sample_2 | isotropic | sample_conditions_2 |
3D HNCA | sample_2 | isotropic | sample_conditions_2 |
3D HNCO | sample_3 | isotropic | sample_conditions_3 |
3D C(CO)NH | sample_3 | isotropic | sample_conditions_3 |
3D HNCACB | sample_3 | isotropic | sample_conditions_3 |
3D HN(CA)CO | sample_3 | isotropic | sample_conditions_3 |
3D CBCA(CO)NH | sample_3 | isotropic | sample_conditions_3 |
2D 1H-15N HSQC | sample_3 | isotropic | sample_conditions_3 |
3D HNCA | sample_3 | isotropic | sample_conditions_3 |
2D 1H-15N HSQC | sample_4 | isotropic | sample_conditions_4 |
3D HNCO | sample_4 | isotropic | sample_conditions_4 |
3D HNCA | sample_4 | isotropic | sample_conditions_4 |
Software:
VNMRj - collection
NMRPipe - processing
CcpNMR - chemical shift assignment
NMR spectrometers:
- Varian VNMRS 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts