BMRB Entry 51257

Name: Denatured State Backbone Assignments for UBA(1) from HHR23A in Guanidine Hydrochloride Solutions
Published: 2022-06-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51257

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Denatured State Backbone Assignments for UBA(1) from HHR23A in Guanidine Hydrochloride Solutions

Deposition date:

2022-01-04

Original release date:

2022-06-08

Authors:

Becht, Dustin; Briknarova, Klara; Bowler, Bruce

Citation:

Citation: Becht, Dustin; Leavens, Moses; Zeng, Baisen; Rothfuss, Michael; Briknarova, Klara; Bowler, Bruce. "Residual Structure in the Denatured State of the Fast-Folding UBA(1) Domain from the Human DNA Excision Repair Protein HHR23A" Biochemistry 61, 767-784 (2022).
PubMed: 35430812

Assembly members:

Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: STLVTGSEYETMLTEIMSMG YERERVVAALRASYNNPHRA VEYLLTGIPG

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	388
15N chemical shifts	188
1H chemical shifts	188

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UBA(1)	1

Entities:

Entity 1, UBA(1) 50 residues - Formula weight is not available

1	SER	THR	LEU	VAL	THR	GLY	SER	GLU	TYR	GLU
2	THR	MET	LEU	THR	GLU	ILE	MET	SER	MET	GLY
3	TYR	GLU	ARG	GLU	ARG	VAL	VAL	ALA	ALA	LEU
4	ARG	ALA	SER	TYR	ASN	ASN	PRO	HIS	ARG	ALA
5	VAL	GLU	TYR	LEU	LEU	THR	GLY	ILE	PRO	GLY

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_2	isotropic	sample_conditions_2
3D HNCA	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_3	isotropic	sample_conditions_3
3D C(CO)NH	sample_3	isotropic	sample_conditions_3
3D HNCACB	sample_3	isotropic	sample_conditions_3
3D HN(CA)CO	sample_3	isotropic	sample_conditions_3
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_3
3D HNCA	sample_3	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_4	isotropic	sample_conditions_4
3D HNCO	sample_4	isotropic	sample_conditions_4
3D HNCA	sample_4	isotropic	sample_conditions_4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 51257

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	SER	THR	LEU	VAL	THR	GLY	SER	GLU	TYR	GLU
2	THR	MET	LEU	THR	GLU	ILE	MET	SER	MET	GLY
3	TYR	GLU	ARG	GLU	ARG	VAL	VAL	ALA	ALA	LEU
4	ARG	ALA	SER	TYR	ASN	ASN	PRO	HIS	ARG	ALA
5	VAL	GLU	TYR	LEU	LEU	THR	GLY	ILE	PRO	GLY

1	SER	THR	LEU	VAL	THR	GLY	SER	GLU	TYR	GLU
2	THR	MET	LEU	THR	GLU	ILE	MET	SER	MET	GLY
3	TYR	GLU	ARG	GLU	ARG	VAL	VAL	ALA	ALA	LEU
4	ARG	ALA	SER	TYR	ASN	ASN	PRO	HIS	ARG	ALA
5	VAL	GLU	TYR	LEU	LEU	THR	GLY	ILE	PRO	GLY

1	SER	THR	LEU	VAL	THR	GLY	SER	GLU	TYR	GLU
2	THR	MET	LEU	THR	GLU	ILE	MET	SER	MET	GLY
3	TYR	GLU	ARG	GLU	ARG	VAL	VAL	ALA	ALA	LEU
4	ARG	ALA	SER	TYR	ASN	ASN	PRO	HIS	ARG	ALA
5	VAL	GLU	TYR	LEU	LEU	THR	GLY	ILE	PRO	GLY