BMRB Entry 51285

Name: Solid-state NMR study reveals that seeding can lead to in-vivo like fibrils for AA amyloidosis
Published: 2022-09-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51285

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR study reveals that seeding can lead to in-vivo like fibrils for AA amyloidosis

Deposition date:

2022-01-20

Original release date:

2022-09-20

Authors:

Sundaria, Arpita; Sarkar, Riddhiman; Liberta, Falk; Haupt, Christian; Savran, Dilan; Schwierz, Nadine; Fandrich, Marcus; Reif, Bernd

Citation:

Citation: Sundaria, Arpita; Liberta, Falk; Savran, Dilan; Sarkar, Riddhiman; Rodina, Natalia; Peters, Carsten; Schwierz, Nadine; Haupt, Christian; Schmidt, Matthias; Reif, Bernd. "SAA fibrils involved in AA amyloidosis are similar in bulk and by single particle reconstitution: A MAS solid-state NMR study" J. Struct. Biol. X 6, 100069-100069 (2022).
PubMed: 35924280

Assembly members:

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMALC2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GFFSFIGEAFQGAGDMWRAY TDMKEAGWKDGDKYFHARGN YDAAQRGPGGVWAAEKISDA RESFQEFFGRGHEDTMADQE ANRHGRSGKDPNYYRPPGLP AKY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	162
15N chemical shifts	13

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SAA 1.1	1

Entities:

Entity 1, SAA 1.1 103 residues - Formula weight is not available

1

GLY

PHE

SER

PHE

ILE

GLY

GLU

ALA

PHE

2

GLN

GLY

ALA

GLY

ASP

MET

TRP

ARG

ALA

TYR

3

THR

ASP

MET

LYS

GLU

ALA

GLY

TRP

LYS

ASP

4

GLY

ASP

LYS

TYR

PHE

HIS

ALA

ARG

GLY

ASN

5

TYR

ASP

ALA

GLN

ARG

GLY

PRO

GLY

6

VAL

TRP

ALA

GLU

LYS

ILE

SER

ASP

ALA

7

ARG

GLU

SER

PHE

GLN

GLU

PHE

GLY

ARG

8

GLY

HIS

GLU

ASP

THR

MET

ALA

ASP

GLN

GLU

9

ALA

ASN

ARG

HIS

GLY

ARG

SER

GLY

LYS

ASP

10

PRO

ASN

TYR

ARG

PRO

GLY

LEU

PRO

11

ALA

LYS

TYR

Samples:

sample_1: SAA 1.1, [U-100% 13C; U-100% 15N], 50 uM; tris buffer 20 mM

sample_conditions_1: pH: 8.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
CONCA	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR vVersion-2 - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz