BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 51285

Title: Solid-state NMR study reveals that seeding can lead to in-vivo like fibrils for AA amyloidosis   PubMed: 35924280

Deposition date: 2022-01-20 Original release date: 2022-09-20

Authors: Sundaria, Arpita; Sarkar, Riddhiman; Liberta, Falk; Haupt, Christian; Savran, Dilan; Schwierz, Nadine; Fandrich, Marcus; Reif, Bernd

Citation: Sundaria, Arpita; Liberta, Falk; Savran, Dilan; Sarkar, Riddhiman; Rodina, Natalia; Peters, Carsten; Schwierz, Nadine; Haupt, Christian; Schmidt, Matthias; Reif, Bernd. "SAA fibrils involved in AA amyloidosis are similar in bulk and by single particle reconstitution: A MAS solid-state NMR study"  J. Struct. Biol. X 6, 100069-100069 (2022).

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMALC2

Entity Sequences (FASTA):
entity_1: GFFSFIGEAFQGAGDMWRAY TDMKEAGWKDGDKYFHARGN YDAAQRGPGGVWAAEKISDA RESFQEFFGRGHEDTMADQE ANRHGRSGKDPNYYRPPGLP AKY

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts13

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SAA 1.11

Entities:

Entity 1, SAA 1.1 103 residues - Formula weight is not available

1   GLYPHEPHESERPHEILEGLYGLUALAPHE
2   GLNGLYALAGLYASPMETTRPARGALATYR
3   THRASPMETLYSGLUALAGLYTRPLYSASP
4   GLYASPLYSTYRPHEHISALAARGGLYASN
5   TYRASPALAALAGLNARGGLYPROGLYGLY
6   VALTRPALAALAGLULYSILESERASPALA
7   ARGGLUSERPHEGLNGLUPHEPHEGLYARG
8   GLYHISGLUASPTHRMETALAASPGLNGLU
9   ALAASNARGHISGLYARGSERGLYLYSASP
10   PROASNTYRTYRARGPROPROGLYLEUPRO
11   ALALYSTYR

Samples:

sample_1: SAA 1.1, [U-100% 13C; U-100% 15N], 50 uM; tris buffer 20 mM

sample_conditions_1: pH: 8.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
CONCAsample_1isotropicsample_conditions_1

Software:

CcpNMR vVersion-2 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz