BMRB Entry 51331

Name: Solid-state NMR 13C and 15N resonance assignment of Vibrio sp. SemiSWEET transporter in lipid bilayers
Published: 2022-05-31

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51331

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solid-state NMR 13C and 15N resonance assignment of Vibrio sp. SemiSWEET transporter in lipid bilayers

Deposition date: 2022-02-17 Original release date: 2022-05-31

Authors: Zhang, Yan; Yang, Jun

Citation: Zhang, Yan; Yang, Jun. "Solid-state NMR 13C and 15N resonance assignment of Vibrio sp. SemiSWEET transporter in lipid bilayers" Biomol. NMR Assignments ., .-..

Assembly members:
entity_1, polymer, 97 residues, Formula weight is not available

Natural source: Common Name: Vibrio sp. Taxonomy ID: 678 Superkingdom: Bacteria Kingdom: not available Genus/species: Vibrio Vibrio sp.

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):
entity_1: MALIERIGKALEPLMLVMGL ISPLATMPQLYKLYVSHSEH ALGLSLTTWLLYSFIALLWT IYGIYHKNPTIWVGNCLGFL MYVAMVVGIIAHTGGTY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	334
15N chemical shifts	91

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VsSemiSWEET, subunit 1	1
2	VsSemiSWEET, subunit 2	1

Entities:

Entity 1, VsSemiSWEET, subunit 1 97 residues - Formula weight is not available

1

MET

ALA

LEU

ILE

GLU

ARG

ILE

GLY

LYS

ALA

2

LEU

GLU

PRO

LEU

MET

LEU

VAL

MET

GLY

LEU

3

ILE

SER

PRO

LEU

ALA

THR

MET

PRO

GLN

LEU

4

TYR

LYS

LEU

TYR

VAL

SER

HIS

SER

GLU

HIS

5

ALA

LEU

GLY

LEU

SER

LEU

THR

TRP

LEU

6

LEU

TYR

SER

PHE

ILE

ALA

LEU

TRP

THR

7

ILE

TYR

GLY

ILE

TYR

HIS

LYS

ASN

PRO

THR

8

ILE

TRP

VAL

GLY

ASN

CYS

LEU

GLY

PHE

LEU

9

MET

TYR

VAL

ALA

MET

VAL

GLY

ILE

10

ALA

HIS

THR

GLY

THR

TYR

Samples:

sample_1: H2O 30 % w/w; the artificially extracted E.coli lipid 28 % w/w; VsSemiSWEET, [U-100% 13C; U-100% 15N], 42 % w/w

sample_conditions_1: pH: 8.0; pressure: 1 atm; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NCA	sample_1	isotropic	sample_conditions_1
2D DARR	sample_1	isotropic	sample_conditions_1
3D NCACX	sample_1	isotropic	sample_conditions_1
3D NCOCX	sample_1	isotropic	sample_conditions_1
3D CONCA	sample_1	isotropic	sample_conditions_1
3D NCACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Varian Uniform NMR System 600 MHz