BMRB Entry 51362
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51362
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Structural basis for tRNA recognition and regulation of METTL1-WDR4 methyltransferase PubMed: 36599985
Deposition date: 2022-03-14 Original release date: 2023-01-13
Authors: Viennet, Thibault; Li, Jiazhi; Gregory, Richard; Arthanari, Haribabu
Citation: Li, Jiazhi; Wang, Longfei; Hahn, Quentin; Nowak, Radoslaw; Viennet, Thibault; Oreliana, Esteban; Burman, Shourya; Yue, Hong; Hunkeler, Moritz; Fontana, Pietro; Wu, Hao; Arthanari, Haribabu; Fischer, Eric; Gregory, Richard. "Structural basis of regulated m7G tRNA modification by METTL1-WDR4" Nature 613, 391-397 (2023).
Assembly members:
entity_1, polymer, 81 residues, 9426.37 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet-1
Entity Sequences (FASTA):
entity_1: SQDPNSMAAETRNVAGAEAP
PPQKRYYRQRAHSNPMADHT
LRYPVKPEEMDWSELYPEFF
APLTQNQSHDDPKDKKEKRA
Q
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 193 |
15N chemical shifts | 60 |
1H chemical shifts | 60 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | METTL1 1-75 | 1 |
Entities:
Entity 1, METTL1 1-75 81 residues - 9426.37 Da.
1 | SER | GLN | ASP | PRO | ASN | SER | MET | ALA | ALA | GLU | ||||
2 | THR | ARG | ASN | VAL | ALA | GLY | ALA | GLU | ALA | PRO | ||||
3 | PRO | PRO | GLN | LYS | ARG | TYR | TYR | ARG | GLN | ARG | ||||
4 | ALA | HIS | SER | ASN | PRO | MET | ALA | ASP | HIS | THR | ||||
5 | LEU | ARG | TYR | PRO | VAL | LYS | PRO | GLU | GLU | MET | ||||
6 | ASP | TRP | SER | GLU | LEU | TYR | PRO | GLU | PHE | PHE | ||||
7 | ALA | PRO | LEU | THR | GLN | ASN | GLN | SER | HIS | ASP | ||||
8 | ASP | PRO | LYS | ASP | LYS | LYS | GLU | LYS | ARG | ALA | ||||
9 | GLN |
Samples:
sample_1: METTL1 1-75, [U-100% 13C; U-100% 15N], 420 mM; NaPi 50 mM; NaCl 100 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6; pressure: 1 atm; temperature: 288 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4.2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
Related Database Links:
HUGO | HGNC:7030 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts