BMRB Entry 51380
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51380
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C and 15N Backbone Chemical Shift Assignments of GTP-bound Rab3a PubMed: 35809645
Deposition date: 2022-03-23 Original release date: 2022-07-12
Authors: Lv, Guohua; Ko, Myung Soo; Das, Tapojyoti; Eliezer, David
Citation: Lv, Guohua; Ko, Myung Soo; Das, Tapojyoti; Eliezer, David. "Molecular and Functional Interactions of Alpha-Synuclein with Rab3a" J. Biol. Chem. 298, 102239-102239 (2022).
Assembly members:
entity_1, polymer, 172 residues, Formula weight is not available
entity_GTP, non-polymer, 523.180 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
entity_1: SDQNFDYMFKILIIGNSSVG
KTSFLFRYADDSFTPAFVST
VGIDFKVKTIYRNDKRIKLQ
IWDTAGLERYRTITTAYYRG
AMGFILMYDITNEESFNAVQ
DWSTQIKTYSWDNAQVLLVG
NKADMEDERVVSSERGRQLA
DHLGFEFFEASAKDNINVKQ
TFERLVDVIAEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 496 |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rab3a_15-186 | 1 |
| 2 | GTP | 2 |
Entities:
Entity 1, Rab3a_15-186 172 residues - Formula weight is not available
| 1 | SER | ASP | GLN | ASN | PHE | ASP | TYR | MET | PHE | LYS | ||||
| 2 | ILE | LEU | ILE | ILE | GLY | ASN | SER | SER | VAL | GLY | ||||
| 3 | LYS | THR | SER | PHE | LEU | PHE | ARG | TYR | ALA | ASP | ||||
| 4 | ASP | SER | PHE | THR | PRO | ALA | PHE | VAL | SER | THR | ||||
| 5 | VAL | GLY | ILE | ASP | PHE | LYS | VAL | LYS | THR | ILE | ||||
| 6 | TYR | ARG | ASN | ASP | LYS | ARG | ILE | LYS | LEU | GLN | ||||
| 7 | ILE | TRP | ASP | THR | ALA | GLY | LEU | GLU | ARG | TYR | ||||
| 8 | ARG | THR | ILE | THR | THR | ALA | TYR | TYR | ARG | GLY | ||||
| 9 | ALA | MET | GLY | PHE | ILE | LEU | MET | TYR | ASP | ILE | ||||
| 10 | THR | ASN | GLU | GLU | SER | PHE | ASN | ALA | VAL | GLN | ||||
| 11 | ASP | TRP | SER | THR | GLN | ILE | LYS | THR | TYR | SER | ||||
| 12 | TRP | ASP | ASN | ALA | GLN | VAL | LEU | LEU | VAL | GLY | ||||
| 13 | ASN | LYS | ALA | ASP | MET | GLU | ASP | GLU | ARG | VAL | ||||
| 14 | VAL | SER | SER | GLU | ARG | GLY | ARG | GLN | LEU | ALA | ||||
| 15 | ASP | HIS | LEU | GLY | PHE | GLU | PHE | PHE | GLU | ALA | ||||
| 16 | SER | ALA | LYS | ASP | ASN | ILE | ASN | VAL | LYS | GLN | ||||
| 17 | THR | PHE | GLU | ARG | LEU | VAL | ASP | VAL | ILE | ALA | ||||
| 18 | GLU | LYS |
Entity 2, GTP - C10 H16 N5 O14 P3 - 523.180 Da.
| 1 | GTP |
Samples:
sample_1: Rab3a_15-186, [U-13C; U-15N; U-2H], 260 uM; GTP 1 mM; Tris 50 mM; NaCl 150 mM; DTT 2 mM; MgCl2 10 mM; NaN3 0.05%
sample_conditions_1: pH: 7.1; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment, peak picking
TOPSPIN - collection
NMRPipe - processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts