BMRB Entry 51390

Name: HN-S CTD
Published: 2022-05-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51390

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: HN-S CTD

Deposition date: 2022-04-06 Original release date: 2022-05-04

Authors: Price, Caitlin; Hussain, Alya; Henen, Morkos; Vogeli, Beat; Sette, Marco; Barbato, Gaetano; Boelens, Rolf

Citation: Price, Caitlin; Hussain, Alya; Henen, Morkos; Vogeli, Beat; Sette, Marco; Barbato, Gaetano; Boelens, Rolf. "Histone-like nucleoid structuring protein interaction with DNA in Shigella flexneri" .

Assembly members:
entity_1, polymer, 48 residues, Formula weight is not available

Natural source: Common Name: Shigella flexneri Taxonomy ID: 623 Superkingdom: Bacteria Kingdom: not available Genus/species: Shigella flexneri

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet28(a)+

Entity Sequences (FASTA):
entity_1: RAQRPAKYSYVDENGETKTW TGQGRTPAVIKKAMDEQGKS LDDFLIKQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	130
15N chemical shifts	41
1H chemical shifts	216

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HN-S CTD	1

Entities:

Entity 1, HN-S CTD 48 residues - Formula weight is not available

1

ARG

ALA

GLN

ARG

PRO

ALA

LYS

TYR

SER

TYR

2

VAL

ASP

GLU

ASN

GLY

GLU

THR

LYS

THR

TRP

3

THR

GLY

GLN

GLY

ARG

THR

PRO

ALA

VAL

ILE

4

LYS

ALA

MET

ASP

GLU

GLN

GLY

LYS

SER

5

LEU

ASP

PHE

LEU

ILE

LYS

GLN

Samples:

sample_1: HN-S CTD, [U-100% 13C; U-100% 15N], 700 uM; NaCl 20 mM

sample_conditions_1: ionic strength: 30 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment, peak picking

TOPSPIN v4.1.1 - collection, data analysis

VNMR - collection

NMRDraw - data analysis, peak picking, processing

NMR spectrometers:

Bruker AVANCE NEO 600 MHz
Varian INOVA 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts