BMRB Entry 51393
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51393
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Title: drosophila pAbp RRM3 PubMed: 36688322
Deposition date: 2022-04-07 Original release date: 2023-07-04
Authors: Hollmann, Nele Merret; Simon, Bernd; Hennig, Janosch
Citation: Hollmann, Nele Merret; Jagtap, Pravin; Linse, Johanna-Barbara; Ullmann, Philip; Payr, Marco; Murciano, Brice; Simon, Bernd; Hub, Jochen; Hennig, Janosch. "Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein" Nucleic Acids Res. 51, 1895-1913 (2023).
Assembly members:
entity_1, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11
Entity Sequences (FASTA):
entity_1: GEKAKLFTNVYVKNFTEDFD
DEKLKEFFEPYGKITSYKVM
SKEDGKSKGFGFVAFETTEA
AEAAVQALNGKDMGEGKSLY
VARAQKKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 167 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 86 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | drosophila pAbp RRM3 | 1 |
Entities:
Entity 1, drosophila pAbp RRM3 88 residues - Formula weight is not available
| 1 | GLY | GLU | LYS | ALA | LYS | LEU | PHE | THR | ASN | VAL | ||||
| 2 | TYR | VAL | LYS | ASN | PHE | THR | GLU | ASP | PHE | ASP | ||||
| 3 | ASP | GLU | LYS | LEU | LYS | GLU | PHE | PHE | GLU | PRO | ||||
| 4 | TYR | GLY | LYS | ILE | THR | SER | TYR | LYS | VAL | MET | ||||
| 5 | SER | LYS | GLU | ASP | GLY | LYS | SER | LYS | GLY | PHE | ||||
| 6 | GLY | PHE | VAL | ALA | PHE | GLU | THR | THR | GLU | ALA | ||||
| 7 | ALA | GLU | ALA | ALA | VAL | GLN | ALA | LEU | ASN | GLY | ||||
| 8 | LYS | ASP | MET | GLY | GLU | GLY | LYS | SER | LEU | TYR | ||||
| 9 | VAL | ALA | ARG | ALA | GLN | LYS | LYS | ALA |
Samples:
sample_1: pAbp RRM3, [U-99% 13C; U-99% 15N], 0.3 mM; sodium azide 0.01%; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5pl7 - collection
NMRPipe - processing
CARA - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts